A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri
文献类型：期刊论文
| 作者 | Wang, Mengqiang1 ; Wang, Baojie1; Jiang, Keyong1; Liu, Mei1; Shi, Xiaowei1,3,4; Wang, Lei1,2
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2018 |
| 卷号 | 72页码:282-290 |
| 关键词 | Chlamys farreri Innate immunity Manganese superoxide dismutase |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2017.11.010 |
| 通讯作者 | Wang, Lei(wanglei@qdio.ac.cn) |
| 英文摘要 | Superoxide dismutase (SOD) ubiquitously found in both prokaryotes and eukaryotes functions as the first and essential enzyme in the antioxidant system. In the present study, a manganese SOD (designated as CfmtMnSOD) was cloned from Zhikong scallop Chlamys farreri. The complete cDNA sequence of CfmtMnSOD contained a 681 bp open reading frame (ORF), encoding a peptide of 226 amino acids. A SOD_Fe_N domain and a SOD_Fe_C domain were found in the deduced amino acid sequence of CfmtMnSOD. The mRNA transcripts of CfmtMnSOD were constitutively expressed in all the tested tissues, including gill, gonad, hepatopancreas, hemocytes, mantle and muscle, with the highest expression level in hemocytes. After the stimulation of Vibrio splendidus, Staphylococcus aureus and Yarrowia lipolytica, the mRNA transcripts of CfmtMnSOD in hemocytes all significantly increased. The purified rCfmtMnSOD protein exhibited Mn2+ dependent specific and low stable enzymatic activities. After Vibrio challenge, the cumulative mortality of CfmtMnSOD-suppressed scallops was significantly higher than those of control groups and the semi-lethal time for CfmtMnSOD-suppressed scallops was rather shorter than those of control groups either. Moreover, the final mortality rate of CfmtMnSOD-suppressed group was significant higher than those of control groups, even without Vibrio challenge. All these results indicated that CfmtMnSOD was efficient antioxidant enzyme involved in the innate immunity, and also essential for the survival of C. farreri. |
| 资助项目 | Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Major Projects of Shandong Province[2015ZDZX05002] ; China Postdoctoral Science Foundation[2017M610427] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000423644600033 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/158004]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Lei |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Linyi Univ, Linyi 276000, Peoples R China 4.Shandong Prov Engn Technol Res Ctr Liman Chinese, Linyi 276000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Mengqiang,Wang, Baojie,Jiang, Keyong,et al. A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:282-290. |
| APA | Wang, Mengqiang,Wang, Baojie,Jiang, Keyong,Liu, Mei,Shi, Xiaowei,&Wang, Lei.(2018).A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,72,282-290. |
| MLA | Wang, Mengqiang,et al."A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 72(2018):282-290. |
入库方式： OAI收割
来源：海洋研究所
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