Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit
文献类型：期刊论文
| 作者 | Wu, Jing1,2,3 ; Chen, Huaxin1,2; Jiang, Peng1,2
|
| 刊名 | BIOENGINEERED
 |
| 出版日期 | 2018 |
| 卷号 | 9期号:1页码:108-115 |
| 关键词 | Allophycocyanin Chromophore attachment Chromophorylation rate Immunofluorescence assay Phycobiliprotein |
| ISSN号 | 2165-5979 |
| DOI | 10.1080/21655979.2017.1321282 |
| 通讯作者 | Chen, Huaxin(chenhx001@126.com) ; Jiang, Peng(Jiangpeng@qdio.ac.cn) |
| 英文摘要 | The fusion protein (SLA) of streptavidin and allophycocyanin subunit (holo-ApcA) was biosynthesized in Escherichia coli by a dual plasmid system. The recombinant SLA, purified by affinity chromatography, showed spectral properties similar to natural allophycocyanin subunit (ApcA). Spectral and Zinc staining analysis indicated that the recombinant SLA covalently bound phycocyanobilin (PCB). To improve chromophorylation rate of recombinant SLA, an in vitro chromophore attachment reaction system was established, which contained partially chromophylated SLA, PCB and lyase CpcS. Spectral analysis showed that PCB bound to the recombinant SLA rapidly during the reaction. The chromophorylation rate of SLA was improved from 21.1% to 86.5%. Immunofluorescence assay showed that SLA with high chromophorylation rate had higher detection signal. Thus, in vitro chromophore attachment is an effective way to improve the chromophorylation rate of recombinant phycobiliprotein. |
| 资助项目 | National Natural Science Foundation[41276164] ; National 863 Program[2014AA093505] ; National 863 Program[2014AA093501] ; development funds of science and technology of Shinan district[2016-3-010-ZH] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000425053900001 |
| 出版者 | TAYLOR & FRANCIS INC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/158038]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Chen, Huaxin; Jiang, Peng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Jing,Chen, Huaxin,Jiang, Peng. Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit[J]. BIOENGINEERED,2018,9(1):108-115. |
| APA | Wu, Jing,Chen, Huaxin,&Jiang, Peng.(2018).Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit.BIOENGINEERED,9(1),108-115. |
| MLA | Wu, Jing,et al."Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit".BIOENGINEERED 9.1(2018):108-115. |
入库方式： OAI收割
来源：海洋研究所
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