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Immunostimulatory effects of sulfated chitosans on RAW 264.7 mouse macrophages via the activation of PI3 K/Akt signaling pathway

文献类型：期刊论文

作者Yang, Yue1,2,3; Xing, Ronge1,2; Liu, Song1,2; Qin, Yukun1,2; Li, Kecheng2; Yu, Huahua1,2; Li, Pengcheng1,2
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2018-03-01
卷号108页码:1310-1321
ISSN号0141-8130
关键词Sulfated chitosan Immunostimulatory activity Nuclear factor-kappa B Toll like receptor-4
DOI10.1016/j.ijbiomac.2017.11.042
通讯作者Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要To investigate the immunostimulatory effects of chitosan sulfates, we prepared alpha- and beta-chitosan sulfates with different molecular weights and compared their immunostimulatory activities in RAW 264.7 macrophages. Results suggest that beta-chitosan sulfates were more active than alpha-chitosan in promoting nitric oxide (NO) production. Further study show that beta-chitosan sulfate significantly promoted the production of NO, prostaglandin E-2, tumor necrosis factor (TNF)-alpha, interleukin-6 and interleukin-1 beta at the levels of transcription and translation. Moreover, Western blots revealed that it induced the phosphorylation of p85 and Akt, and the nuclear translocation of p50/p65 and c-Fos/c-Jun. The luciferase activity of cells pretreated with I3-chitosan sulfate further confirmed the nuclear translocation of p50/p65 and c-Fosic-Jun. Determination of Toll-like receptor (TLR) 4 expression suggested that beta-chitosan sulfate at least partly bound to TLR4. In conclusion, beta-chitosan sulfates activate RAW 264.7 cells through the PI3K-Akt pathway, which is dependent on activator protein-1 and nuclear factor-kappa B activation. (C) 2017 Elsevier B.V. All rights reserved.
资助项目Qingdao science and technology plan[14-2-3-47-nsh] ; Shandong Provincial Science and Technology Major Project, China[2015ZDZX05003] ; Science and Technology Development Program of Shandong Province[2014GGH215006] ; commonweal item of State Oceanic Administration People's Republic of China[201405038-2] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
出版者ELSEVIER SCIENCE BV
WOS记录号WOS:000425071500149
源URL[http://ir.qdio.ac.cn/handle/337002/158056]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Xing, Ronge; Li, Pengcheng
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Yue,Xing, Ronge,Liu, Song,et al. Immunostimulatory effects of sulfated chitosans on RAW 264.7 mouse macrophages via the activation of PI3 K/Akt signaling pathway[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,108:1310-1321.
APA Yang, Yue.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2018).Immunostimulatory effects of sulfated chitosans on RAW 264.7 mouse macrophages via the activation of PI3 K/Akt signaling pathway.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,108,1310-1321.
MLA Yang, Yue,et al."Immunostimulatory effects of sulfated chitosans on RAW 264.7 mouse macrophages via the activation of PI3 K/Akt signaling pathway".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 108(2018):1310-1321.

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来源：海洋研究所

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