A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection
文献类型：期刊论文
| 作者 | Yang, Feifei1; Li, Shihao1; Li, Fuhua1,2; Xiang, Jianhai1,2
|
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 81页码:303-311 |
| 关键词 | Cuticle protein WSSV infection VP24 Litopenaeus vannamei |
| ISSN号 | 0145-305X |
| DOI | 10.1016/j.dci.2017.12.018 |
| 通讯作者 | Li, Fuhua(fhli@qdio.ac.cn) |
| 英文摘要 | White spot syndrome virus (WSSV) is a major viral pathogen in global shrimp farming, causing huge economic damage. Through penetrating the outer surface of the target tissues, WSSV enters into the cells of the target tissue to complete the replication process in the host. In the present study, a cuticle protein gene from Litopenaeus vannamei, designated as LvAMP13.4, was identified and proved to be involved in WSSV invasion. The deduced amino acid sequence of LvAMP13.4 contained a signal peptide and a conserved chitin-binding domain type 4 (ChBD4). This cuticle protein gene was mainly expressed in stomach, gill and epidermis. The expression level of LvAMP13.4 was significantly changed during WSSV infection. Silencing of LvAMP13.4 by dsRNA interference apparently reduced the mortality rate and the WSSV copy number in shrimp upon WSSV infection. Furthermore, yeast two-hybrid system and Co-IP assay were performed to confirm that LvAMP13.4 could interact with the major envelop protein VP24 of WSSV. These data indicated that LvAMP13.4 was involved in the invasion process of WSSV through interaction with VP24. The present results could provide new insights for us in understanding the role of host cuticle proteins during virus invasion. (C) 2017 Elsevier Ltd. All rights reserved. |
| 资助项目 | NSFC-ISF Research Program of National Natural Science Foundation of China[31461143007] ; China Agriculture Research System-48[CARS-48] ; China Postdoctoral Science Foundation[2017M612361] |
| WOS研究方向 | Fisheries ; Immunology ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000424726900031 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/158063]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Fuhua |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Feifei,Li, Shihao,Li, Fuhua,et al. A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,81:303-311. |
| APA | Yang, Feifei,Li, Shihao,Li, Fuhua,&Xiang, Jianhai.(2018).A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,81,303-311. |
| MLA | Yang, Feifei,et al."A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 81(2018):303-311. |
入库方式： OAI收割
来源：海洋研究所
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