中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 海洋研究所
  3. 中国科学院海洋研究所
  4. 实验海洋生物学重点实验室
Comparative study of the effect of different fucoidans from Sargassum maclurei and Saccharina japonica on FGFs/FGFR signaling activation in BaF3 cells

文献类型：期刊论文

作者Geng, Lihua1,2,3; Hou, Ningning1,2,3; Zhang, Meng4; Xu, Yingjie4; Zhang, Qi1,2,3; Wang, Jing1; Zhang, Lijuan4; Zhang, Quanbin1,3
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2018-02-01
卷号107页码:2429-2435
关键词Fucoidan Sulfated polysaccharides Fibroblast growth factor (FGF) Fibroblast growth factor receptor (FGFR)
ISSN号0141-8130
DOI10.1016/j.ijbiomac.2017.10.122
通讯作者Zhang, Lijuan(zhanglj@qduhospital.cn) ; Zhang, Quanbin(qbzhang@qdio.ac.cn)
英文摘要Different sulfated polysaccharides have distinct abilities to activate specific fibroblast growth factor (FGF) signaling pathways in FGFR1c-expressing BaF3 cells. In the current study, we first isolated and characterized different fucoidan fractions from Sargassum maclurei and Saccharina japonica. All of the fucoidan fractions were incubated with BaF3 cells in the presence of FGF-1, -2, -7, -8, -9, and -10, respectively, to evaluate their FGFs/FGFR1c signal-activating ability. Our data showed that low molecular weight fucoidan fraction from S. japonica with highest sulfate content (LMWF-2M) had the most potent activity among all of the six tested FGFs. Low sulfated heteropolysacchairde fraction LMWF-0.5M, along with SMP-1, SMP-D-1, and SMP-A-1, only activated the FGF-2/FGFR1c pair (P<0.05). In contrast, SMP, SMP-A, and SMP-D stimulated BaF3 cell proliferation except for FGF-8. Both LMWF-1M and LMWF-2M facilitated FGF-1-, -2-, -8-, and -9-based signaling. The sulfate content was the major contributing factor to the observed activity followed by the molecular weight. The monosaccharide composition also affected the activity, in that SMP and its derivatives with varied monosaccharide composition could not induce BaF3 cell proliferation in the presence of FGF-8. The structure-activity relationships revealed in current study provided useful information for the potential application of fucoidans in FGF/FGFR signaling regulation. (C) 2017 Published by Elsevier B.V.
资助项目Special Fund for Marine Scientific Research in the Public Interest[201405040] ; Natural Science Foundation of China[81672585] ; Key Technology Fund of Shandong Province[2016ZDJS07A07] ; Key Research and Development Project of Shandong Province[2016YYSP002] ; Key Research and Development Project of Shandong Province[2016GSF115031] ; Youth Innovation Promotion Association of CAS[2016190]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:000424062300117
出版者ELSEVIER SCIENCE BV
源URL[http://ir.qdio.ac.cn/handle/337002/158078]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Zhang, Lijuan; Zhang, Quanbin
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Tech, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Qingdao Univ, Inst Cerebrovasc Dis, Affiliated Hosp, Qingdao 266003, Peoples R China
推荐引用方式
GB/T 7714		 Geng, Lihua,Hou, Ningning,Zhang, Meng,et al. Comparative study of the effect of different fucoidans from Sargassum maclurei and Saccharina japonica on FGFs/FGFR signaling activation in BaF3 cells[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,107:2429-2435.
APA Geng, Lihua.,Hou, Ningning.,Zhang, Meng.,Xu, Yingjie.,Zhang, Qi.,...&Zhang, Quanbin.(2018).Comparative study of the effect of different fucoidans from Sargassum maclurei and Saccharina japonica on FGFs/FGFR signaling activation in BaF3 cells.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,107,2429-2435.
MLA Geng, Lihua,et al."Comparative study of the effect of different fucoidans from Sargassum maclurei and Saccharina japonica on FGFs/FGFR signaling activation in BaF3 cells".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 107(2018):2429-2435.

入库方式： OAI收割

来源：海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。