Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda
文献类型：期刊论文
| 作者 | Sun, Yuying1; Zhang, Jiquan1,2,3; Xiang, Jianhai2,3
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 75页码:115-123 |
| 关键词 | Exopalaemon carinicauda Chitin deacetylase Pichia pastoris |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2018.02.004 |
| 通讯作者 | Zhang, Jiquan(zhangjiquan@qdio.ac.cn) |
| 英文摘要 | Chitin deacetylase (CDA, EC 3.5.1.41), belonging to a family of extracellular chitin-modifying enzymes, can catalyze the deacetylation of chitin. In this study, the full-length cDNA sequence encoding chitin deacetylase 1 (EcCDA1) was obtained from Exopalaemon carinicauda. The complete nucleotide sequence of EcCDA1 contained a 1611 bp open reading frame (ORF) encoding EcCDA1 precursor of 536 amino acids. The domain architecture of the deduced EcCDA1 protein contained a signal peptide, a chitin-binding peritrophin-A domain (ChtBD2), a low density lipoprotein receptor class A domain (LDLa) and a Polysacc_deac_1 domain. EcCDA1 mRNA was predominantly expressed in the gills. The expression of EcCDA1 in the prawns challenged with Vibrio parahaemolyticus and Aeromonas hydrophila changed in a time-dependent manner. The expression of EcCDA1 in the prawns challenged with V. parahaemolyticus was up-regulated at 12 h (p < 0.05), and significantly up-regulated at 24 h and 48 h (p < 0.01), and then returned to the control levels at 96 h post-challenge (p > 0.05). At the same time, the expression in Aeromonas-challenged group was significantly up-regulated at 12, 24 and 48 h (p < 0.01) and returned to the control levels at 120h post-challenge (p > 0.05). Then, EcCDA1 was recombinantly expressed in Pichia pastoris and the purified recombinant EcCDA1 could not inhibit the growth of V. parahaemolyticus or A. hydrophila, which indicated that the CDA1 may play its biological activity in immune defense by deacetylation from chitin. |
| 资助项目 | National Natural Science Foundation of China[31172449] ; National Natural Science Foundation of China[41376165] ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; National High Technology Research and Development Program of China[2012AA10A401] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000428824900014 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/158740]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, Jiquan |
| 作者单位 | 1.Hebei Univ, Coll Life Sci, Baoding 071002, Hebei, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yuying,Zhang, Jiquan,Xiang, Jianhai. Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda[J]. FISH & SHELLFISH IMMUNOLOGY,2018,75:115-123. |
| APA | Sun, Yuying,Zhang, Jiquan,&Xiang, Jianhai.(2018).Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda.FISH & SHELLFISH IMMUNOLOGY,75,115-123. |
| MLA | Sun, Yuying,et al."Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda".FISH & SHELLFISH IMMUNOLOGY 75(2018):115-123. |
入库方式： OAI收割
来源：海洋研究所
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