Bacterial communities associated with Shinkaia crosnieri from the Iheya North, Okinawa Trough: Microbial diversity and metabolic potentials
文献类型：期刊论文
| 作者 | Zhang, Jian1,2; Zeng, Zhi-gang3; Chen, Shuai3; Sun, Li1,2 |
| 刊名 | JOURNAL OF MARINE SYSTEMS
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 180页码:228-236 |
| ISSN号 | 0924-7963 |
| 关键词 | Shinkaia crosnieri 165 rRNA gene Microbial community Metabolism Sulfur oxidization |
| DOI | 10.1016/j.jmarsys.2017.02.011 |
| 通讯作者 | Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | Shinkaia crosnieri is a galatheid crab endemic to the deep-sea hydrothermal systems in the Okinawa Trough. In this study, we systematically analyzed and compared the diversity and metabolic potentials of the microbial communities in different tissues (setae, gill, and intestine) of S. crosnieri by high-throughput sequencing technology and quantitative real-time polymerase chain reaction. Sequence analysis based on the V3-V4 regions of the 16S rRNA gene obtained 408,079 taxon tags, which covered 15 phyla, 22 classes, 32 orders, 42 families, and 25 genera. Overall, the microbial communities in all tissues were dominated by Epsilonproteobacteria and Gammaproteobacteria, of which Epsilonproteobacteria was the largest class and accounted for 85.24% of the taxon tags. In addition, 20 classes of bacteria were discovered for the first time to be associated with S. crosnieri and no archaea were detected. Comparative analysis showed that (i) bacteria from different tissues fell into different groups by beta-diversity analysis, (ii) bacterial communities in intestine were similar to that in gill and much more diverse than that in setae, and the sulfur-oxidizing genus Sulfurovum was markedly enriched in intestine and gill. Furthermore, bacteria potentially involved in methane, nitrogen, and metal metabolisms were detected in all samples. The key genes of aprA/dsrA and pmoA involved in sulfate reducing and methane oxidization, respectively, were detected in the gill and gut communities for the first time, and pmoA was significantly more abundant in gill and setae than in intestine. These results provide the first comparative and relatively complete picture of the diversity and metabolic potentials of the bacteria in different tissues of S. crosnieri. These results also indicate that the composition of the microbial communities in hydrothermal fauna changes with time, suggesting the importance of environmental influence. (C) 2017 Elsevier B.V. All rights reserved. |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11030401] ; Strategic Biological Resources Service Network Plan of Chinese Academy of Sciences[ZSSD-005] ; National Basic Research Program of China[2013CB429700] ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Geology ; Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000429514700022 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/158770]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋地质与环境重点实验室 |
| 通讯作者 | Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jian,Zeng, Zhi-gang,Chen, Shuai,et al. Bacterial communities associated with Shinkaia crosnieri from the Iheya North, Okinawa Trough: Microbial diversity and metabolic potentials[J]. JOURNAL OF MARINE SYSTEMS,2018,180:228-236. |
| APA | Zhang, Jian,Zeng, Zhi-gang,Chen, Shuai,&Sun, Li.(2018).Bacterial communities associated with Shinkaia crosnieri from the Iheya North, Okinawa Trough: Microbial diversity and metabolic potentials.JOURNAL OF MARINE SYSTEMS,180,228-236. |
| MLA | Zhang, Jian,et al."Bacterial communities associated with Shinkaia crosnieri from the Iheya North, Okinawa Trough: Microbial diversity and metabolic potentials".JOURNAL OF MARINE SYSTEMS 180(2018):228-236. |
入库方式： OAI收割
来源：海洋研究所
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