Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205)
文献类型：期刊论文
| 作者 | Zhao, Chunyan1,2,3 ; Liu, Qinghua1,3; Xu, Shihong1,3; Xiao, Yongshuang1,3; Wang, Wenqi4; Yang, Jingkun4; Yang, Yang1,2,3; Wang, Yanfeng1,3; Song, Zongcheng5; Li, Jun1,3
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| 刊名 | THERIOGENOLOGY
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| 出版日期 | 2018-06-01 |
| 卷号 | 113页码:137-145 |
| 关键词 | Turbot Lymphocyte antigen 75 (ly75) Testis Spermatogonia Gonadal development |
| ISSN号 | 0093-691X |
| DOI | 10.1016/j.theriogenology.2017.12.016 |
| 通讯作者 | Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| 英文摘要 | Turbot (Schophthalmus maximus) is one of the most important economic marine flatfish species. However, due to rapid development of the industry, genetic resource recession has brought down the efficiency of aquaculture. Therefore, conservation of the genetic resource is increasingly demanded. Recent research proved that type A spermatogonia possesses the properties of spermatogonia stem cell, and it might provide an ideal solution. Therefore, it is necessary to develop an appropriate molecular marker on type A spermatogonia to further isolate and purify of type A spermatogonia in turbot. In this study, turbot lymphocyte antigen 75 (smly75) gene was identified and its localizations of expressions and the temporal transcription patterns were evaluated qualitatively and semiquantitatively. Investigation in testes of development of spermatogonia showed that smly75 mRNA, contrast with vasa and dnd mRNA, was exclusively localized in type A spermatogonia and not detected in type B spermatogonia, spermatocytes or gonadal somatic cells by in situ hybridization. Thus, the smly75 could be a new and convincing molecular marker on identification of type A spermatogonia. In addition, specifically to development pattern of type A spermatogonia, from 7- to 14- month testes, spermatogonia were dominated and the number of type A spermatogonia was increased, corresponding that smly75 expression was up-regulated gradually, while, in 16 month testes, accompanied by that several spermatogonia differentiated into primary spermatocytes, the smly75 expression down-regulated. Finally, broaden in the whole reproductive cycle, the smly75 transcription significantly variated with the differentiation of germ cells and in accordance with the number of type A spermatogonia. It is suggested that testes from 8 to 14 month old males could be used for further isolation and purification of type A-SG. These results will not only help to better understand type A spermatogonia, but also further facilitate type A spematogonia-mediated germ cell manipulation in turbot. (C) 2018 Elsevier Inc. All rights reserved. |
| 资助项目 | National Natural Science Foundation of China[31372514] ; National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; Modern Agro-Industry Technology Research System[nycytx-50] ; China Postdoctoral Science Foundation[2014M551973] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS ; Chinese Academy of science and technology service network planning[KFJ-EW-STS-060] |
| WOS研究方向 | Reproductive Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000431091900018 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/158966]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Qinghua; Li, Jun |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Peoples R China 5.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Chunyan,Liu, Qinghua,Xu, Shihong,et al. Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205)[J]. THERIOGENOLOGY,2018,113:137-145. |
| APA | Zhao, Chunyan.,Liu, Qinghua.,Xu, Shihong.,Xiao, Yongshuang.,Wang, Wenqi.,...&Li, Jun.(2018).Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205).THERIOGENOLOGY,113,137-145. |
| MLA | Zhao, Chunyan,et al."Identification of type A spermatogonia in turbot (Scophthalmus maximus) using a new cell-surface marker of Lymphocyte antigen 75 (ly75/CD205)".THERIOGENOLOGY 113(2018):137-145. |
入库方式： OAI收割
来源：海洋研究所
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