The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies
文献类型：期刊论文
| 作者 | Han, Yu1,2,3; Zang, Kun1,2,3; Liu, Changshui1,2; Li, Yingjie1,2; Ma, Qingjun1,2 |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
 |
| 出版日期 | 2018-05-01 |
| 卷号 | 74页码:283-287 |
| 关键词 | Vibrio anguillarum siderophore-interacting proteins iron release siderophores |
| ISSN号 | 2053-230X |
| DOI | 10.1107/S2053230X18005125 |
| 通讯作者 | Ma, Qingjun(qma@qdio.ac.cn) |
| 英文摘要 | Siderophore-interacting proteins (SIPs) play an important role in iron acquisition in many bacteria. SIPs release iron from the internalized ferric siderophore complex by reducing ferric iron to ferrous iron, but how the iron is reduced is not well understood. Here, a sip gene was identified in the genome of Vibrio anguillarum 775. To further understand the catalytic mechanism of the protein, the SIP was overexpressed in Escherichia coli Rosetta (DE3) cells, purified and crystallized for X-ray diffraction analysis. The crystal diffracted to 1.113 angstrom resolution and belonged to space group P2(1), with unit-cell parameters a = 64.63, b = 58.47, c = 70.65 angstrom, beta= 114.19 degrees. |
| 资助项目 | 1000 Talents Program of China ; 100 Talents Program of the Chinese Academy of Sciences ; AoShan Talents Program of Qingdao National Laboratory for Marine Science and Technology[2015ASTP] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 语种 | 英语 |
| WOS记录号 | WOS:000431249400003 |
| 出版者 | INT UNION CRYSTALLOGRAPHY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/158986]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Ma, Qingjun |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Yu,Zang, Kun,Liu, Changshui,et al. The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2018,74:283-287. |
| APA | Han, Yu,Zang, Kun,Liu, Changshui,Li, Yingjie,&Ma, Qingjun.(2018).The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,74,283-287. |
| MLA | Han, Yu,et al."The putative siderophore-interacting protein from Vibrio anguillarum: protein production, analysis, crystallization and X-ray crystallographic studies".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 74(2018):283-287. |
入库方式： OAI收割
来源：海洋研究所
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