Glucofucogalactan, a heterogeneous low-sulfated polysaccharide from Saccharina japonica and its bioactivity
文献类型：期刊论文
| 作者 | Geng, Lihua1,2,3; Zhang, Quanbin1,3; Wang, Jing1,3; Jin, Weihua4; Zhao, Tingting5; Hu, Weicheng6 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 113页码:90-97 |
| 关键词 | Saccharina japonica Heteropolysaccharide Aristolochic acid Immunomodulation |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2018.02.008 |
| 通讯作者 | Zhang, Quanbin(qbzhang@qdio.ac.cn) |
| 英文摘要 | Crude polysaccharide obtained from Saccharina japonica using acid hydrolysis and precipitation was separated into sulfated fuco-oligosaccharide (HDF1) and heteropolysaccharide (HDF2). To further explore the bioactive fraction, HDF2 was successfully separated using membrane filtration into HDF2A and HDF2B, which differed in chemical composition and molecular weight. The bioactivity of all the fractions was tested in vitro, including immunomodulatory activity in RAW 264.7 cells and the protective activity in aristolochic acid (AA)-induced NRK-52E cell injury. HDF1 and HDF2B (low-molecular weight sulfated fucans/fuco-oligosaccharides) did not increase the nitric oxide production in RAW 264.7 cells, whereas HDF2 and HDF2A exhibited potential immunomodulatory activity. All the tested compounds showed different degrees of protective activity in AA-induced injury; HDF2A exhibited superior protective activity. Through chemical analysis, HPLC analysis, and IR spectroscopy and MS, it was determined that HDF2A was a galactose-enriched heteropolysaccharide- glucofucogalactan with a distinctive 2:1 ratio of galactose to fucose, In addition, HDF2A also contained a high amount of glucose and minor amounts of mannose, rhamnose, and xylose, with a low content of sulfate. Thus, HDF2A, a complex heterogeneous polysaccharide mixture with a unique monosaccharide composition, could be studied for further structural characterization and pharmaceutical applications. (C) 2018 Elsevier B.V. All rights reserved. |
| 资助项目 | Natural Science Foundation of China[41376166] ; Natural Science Foundation of China[41406144] ; Key Research and Development Project of Shandong Province[2016YYSP002] ; Key Research and Development Project of Shandong Province[2016GSF115031] ; Youth Innovation Promotion Association of CAS[2016190] ; Science and Technology project of Fujian Province[201773015] ; K.C. Wong Education Foundation |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000432503100012 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/159150]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, Quanbin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Tech, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Hangzhou 310014, Zhejiang, Peoples R China 5.China Japan Friendship Hosp, Inst Clin Med Sci, Beijing Key Lab Immune Mediated Inflammatory Dis, Beijing, Peoples R China 6.Huaiyin Normal Univ, Jiangsu Collaborat Innovat Ctr Reg Modern Agr & E, Jiangsu Key Lab Ecoagr Biotechnol Around Hongze L, Huaian 223300, Peoples R China |
| 推荐引用方式 GB/T 7714 | Geng, Lihua,Zhang, Quanbin,Wang, Jing,et al. Glucofucogalactan, a heterogeneous low-sulfated polysaccharide from Saccharina japonica and its bioactivity[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,113:90-97. |
| APA | Geng, Lihua,Zhang, Quanbin,Wang, Jing,Jin, Weihua,Zhao, Tingting,&Hu, Weicheng.(2018).Glucofucogalactan, a heterogeneous low-sulfated polysaccharide from Saccharina japonica and its bioactivity.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,113,90-97. |
| MLA | Geng, Lihua,et al."Glucofucogalactan, a heterogeneous low-sulfated polysaccharide from Saccharina japonica and its bioactivity".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 113(2018):90-97. |
入库方式： OAI收割
来源：海洋研究所
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