First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent
文献类型：期刊论文
| 作者 | Jin, Qian-wen1,2,3; Sun, Qing-lei1,2 ; Zhang, Jian1,2; Sun, Li1,2
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| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2018-09-01 |
| 卷号 | 86页码:17-25 |
| 关键词 | C-type lectin Alaysia sp. Deep-sea hydrothermal vent Antibacterial Hemagglutination |
| ISSN号 | 0145-305X |
| DOI | 10.1016/j.dci.2018.04.019 |
| 通讯作者 | Sun, Qing-lei(lsun@qdio.ac.cn) |
| 英文摘要 | C-type lectins (CTLs) play an important role in innate immune defense. In this study, we identified and characterized two CTLs (Lec1 and Lec2) from the tubeworm Alaysia sp. collected from a hydrothermal vent in Pacmanus. Lec1 and Lec2 possess the typical CTL domain but share low sequence identities (10.8% -20.4%) with known CTLs. Recombinant (r) of Lec1 and Lec2 bound to various PAMPs and a wide arrange of bacteria from neritic and deep-sea environments in a Ca2+-independent manner, but only rLec1 caused agglutination of the bound bacteria. The activities of rLec1 and rLec2 were most stable and highest at 4 degrees C, the ambient temperature of the hydrothermal vent, and decreased at higher temperatures. Both lectins inhibited bacterial growth in a highly selective manner and agglutinated the erythrocytes of fish, rabbit, and chicken in a Ca2+-dependent manner. These results provided the first insights into the functional properties of CTLs in deep-sea Alaysia sp. (C) 2018 Elsevier Ltd. All rights reserved. |
| 资助项目 | Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA11030401] ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Fisheries ; Immunology ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000435060900003 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/159362]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Qing-lei |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin, Qian-wen,Sun, Qing-lei,Zhang, Jian,et al. First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,86:17-25. |
| APA | Jin, Qian-wen,Sun, Qing-lei,Zhang, Jian,&Sun, Li.(2018).First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,86,17-25. |
| MLA | Jin, Qian-wen,et al."First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 86(2018):17-25. |
入库方式： OAI收割
来源：海洋研究所
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