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Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda

文献类型：期刊论文

作者Lv, Xinjia1,2,3; Li, Shihao1,2; Zhang, Chengsong1,2; Xiang, Jianhai1,2; Li, Fuhua1,2
刊名MARINE DRUGS
出版日期2018-05-01
卷号16期号:5页码:13
关键词anti-lipopolysaccharide factor antimicrobial RNA interference flora balance crustacean
ISSN号1660-3397
DOI10.3390/md16050145
通讯作者Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
英文摘要As a kind of antimicrobial peptides (AMP) in crustacean, anti-lipopolysaccharide factors (ALFs) have broad spectrum antimicrobial activities. In the present study, we identified four ALF genes, EcALF2-5, from the ridgetail prawn Exopalaemon carinicauda. Tissue distribution analysis showed that EcALF2 and EcALF4 transcripts were mainly located in gill, epidermis, and stomach, while EcALF3 and EcALF5 were mainly in hemocytes. Peptides corresponding to the LPS binding domain (LBD) of EcALFs were synthesized for analyzing their antimicrobial activities. Minimal inhibitory concentration (MIC) analysis showed that the synthetic LBD peptides of EcALF3 and EcALF4 could inhibit the growth of Gram-positive and Gram-negative bacteria, while the synthetic LBD peptides of EcALF2 and EcALF5 showed antibacterial activity against Vibrio. Incubation of white spot syndrome virus (WSSV) with the synthetic LBD peptides of EcALF3, EcALF4, and EcALF5 could reduce the in vivo viral copy number in WSSV-infected prawns. After silencing of EcALFs, Vibrio exhibited a rapid proliferation in the hepatopancreas of the prawn. The present data showed the important function of different EcALFs in modulating the in vivo bacterial and viral propagation in E. carinicauda. This study will provide new clues into the disease control in aquaculture.
资助项目General Program of National Natural Science Foundation of China[41376165] ; General Program of National Natural Science Foundation of China[41776158] ; General Program of National Natural Science Foundation of China[31772880] ; China Agriculture Research system-48 (CARS-48)
WOS研究方向Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000435195100008
出版者MDPI
源URL[http://ir.qdio.ac.cn/handle/337002/159503]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Li, Shihao; Li, Fuhua
作者单位1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Lv, Xinjia,Li, Shihao,Zhang, Chengsong,et al. Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda[J]. MARINE DRUGS,2018,16(5):13.
APA Lv, Xinjia,Li, Shihao,Zhang, Chengsong,Xiang, Jianhai,&Li, Fuhua.(2018).Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda.MARINE DRUGS,16(5),13.
MLA Lv, Xinjia,et al."Multiple Isoforms of Anti-Lipopolysaccharide Factors and Their Antimicrobial Functions in the Ridgetail Prawn Exopalaemon carinicauda".MARINE DRUGS 16.5(2018):13.

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来源：海洋研究所

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