Molecular characterisation and biochemical properties of phosphomannomutase/phosphoglucomutase (PMM/PGM) in the brown seaweed Saccharina japonica
文献类型：期刊论文
| 作者 | Zhang, Pengyan1,2,3 ; Shao, Zhanru1,2; Li, Lu1,2,3; Liu, Shun1,2,3; Yao, Jianting1,2; Duan, Delin1,2,4
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| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
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| 出版日期 | 2018-08-01 |
| 卷号 | 30期号:4页码:2687-2696 |
| 关键词 | Phosphomannomutase/phosphoglucomutase Saccharina japonica Alginate Fucoidan Biochemical property |
| ISSN号 | 0921-8971 |
| DOI | 10.1007/s10811-018-1460-z |
| 英文摘要 | Saccharina japonica is one of the most important seaweed resources for alginate and fucoidan extraction, and these extracted chemical products are used in foods, textiles, cosmetics, pharmaceuticals, and other applications. The synthesis pathways of alginate and fucoidan are complicated and most of genes and their functions are not clearly understood. Phosphomannomutase is an important enzyme that is involved in the synthesis of both alginate and F-fucoidan in the kelp and also fulfils a phosphoglucomutase function in polysaccharide synthesis. In this study, the full sequence of phosphomannomutase/phosphoglucomutase gene (Sjpmm/Sjpgm) was isolated from S. japonica and characterised. The open reading frame (ORF) of Sjpmm/Sjpgm was 756 bp long, encoded 252 amino acids, and belonged to the haloacid dehydrogenase (HAD) superfamily. The fusion enzyme SjPMM/SjPGM was expressed and purified, and its phosphomannomutase (PMM) and phosphoglucomutase (PGM) activities were determined. The optimal temperature for both PMM and PGM activities was 30 A degrees C, and the optimal pH values were 7.5 and 7.0, respectively. The influences of Mg2+, Mn2+, Co2+, and Ni2+ on the activation of SjPMM/SjPGM were measured, and the maximum activity occurred with Mg2+. Under the optimal conditions, the K (m) values for the substrates glucose-1-phosphate (G1P) and mannose-1-phosphate (M1P) were 0.08 and 1.15 mM, respectively. In addition, high-temperature (25 A degrees C) and high-light intensity (55 +/- 5 mu mol photons m(-2) s(-1)) both significantly increased the transcription of Sjpmm/Sjpgm (up to 1.7-fold and 4.6-fold, respectively). These results implied that the SjPMM/SjPGM was closely related to the synthesis of alginate and F-fucoidan and was directly involved in the acclimatisation of S. japonica to light and temperature stress. |
| 资助项目 | Shandong Key Sci-Technology Research Project[2016ZDJS06B2] ; Shandong Key Sci-Technology Research Project[2016ZDJS06B2] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Key Technology Research and Development Program[2013BAB01B01] ; National Key Technology Research and Development Program[2013BAB01B01] ; Ocean Public Welfare Scientific Research Project[201405040] ; Ocean Public Welfare Scientific Research Project[201405040] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000440782100047 |
| 出版者 | SPRINGER |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/159670]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Duan, Delin |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.State Key Lab Seaweed Bioact Subst, Qingdao 266000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Pengyan,Shao, Zhanru,Li, Lu,et al. Molecular characterisation and biochemical properties of phosphomannomutase/phosphoglucomutase (PMM/PGM) in the brown seaweed Saccharina japonica[J]. JOURNAL OF APPLIED PHYCOLOGY,2018,30(4):2687-2696. |
| APA | Zhang, Pengyan,Shao, Zhanru,Li, Lu,Liu, Shun,Yao, Jianting,&Duan, Delin.(2018).Molecular characterisation and biochemical properties of phosphomannomutase/phosphoglucomutase (PMM/PGM) in the brown seaweed Saccharina japonica.JOURNAL OF APPLIED PHYCOLOGY,30(4),2687-2696. |
| MLA | Zhang, Pengyan,et al."Molecular characterisation and biochemical properties of phosphomannomutase/phosphoglucomutase (PMM/PGM) in the brown seaweed Saccharina japonica".JOURNAL OF APPLIED PHYCOLOGY 30.4(2018):2687-2696. |
入库方式： OAI收割
来源：海洋研究所
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