A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)
文献类型：期刊论文
| 作者 | Yang Yang1,2,3 ; Liu Qinghua1,2; Ma Daoyuan1,2; Song Zongcheng4; Li Jun1,2
|
| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2018-07-01 |
| 卷号 | 36期号:4页码:1342-1348 |
| 关键词 | Ly75/CD205 Japanese flounder Paralichthys olivaceus gonads germ cell-specific marker |
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-019-7390-z |
| 通讯作者 | Liu Qinghua(qinghualiu@qdio.ac.cn) ; Li Jun(junli@qdio.ac.cn) |
| 英文摘要 | Some germ cell marker genes, such as vasa, nanos, and dead end (dnd), have been identified in fish. Recently, lymphocyte antigen 75 (Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder Paralichthys olivaceus ly75 homolog (ly75) was cloned and its expression pattern in gonads was analyzed. The full-length cDNA of ly75 was 7 346 bp, with an open reading frame (ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 kDa. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes. |
| 资助项目 | National Natural Science Foundation of China[31372514] ; National Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; China Agriculture Research System[CARS-47] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Youth Innovation Promotion Association CAS ; Chinese Academy of Science and Technology Service Network Planning[KFJ-EW-STS-060] |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:000441231100028 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/159940]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu Qinghua; Li Jun |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang Yang,Liu Qinghua,Ma Daoyuan,et al. A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(4):1342-1348. |
| APA | Yang Yang,Liu Qinghua,Ma Daoyuan,Song Zongcheng,&Li Jun.(2018).A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205).JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(4),1342-1348. |
| MLA | Yang Yang,et al."A potential germ cell-specific marker in Japanese flounder, Paralichthys olivaceus: identification and characterization of lymphocyte antigen 75 (Ly75/CD205)".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.4(2018):1342-1348. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。