Molecular characterization of the dual oxidase (LvDuox) gene from the pacific white shrimp Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Chen, Y.1,2,3 ; Wang, B. J.1,2; Wang, M. Q.1,2; Liu, M.1,2; Jiang, K. Y.1,2; Wang, L.1,2
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| 刊名 | ISJ-INVERTEBRATE SURVIVAL JOURNAL
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| 出版日期 | 2018 |
| 卷号 | 15页码:316-326 |
| 关键词 | Litopenaeus vannamei Duox antioxidant gene innate immunity |
| ISSN号 | 1824-307X |
| 通讯作者 | Wang, L.(wanglei@qdio.ac.cn) |
| 英文摘要 | The reactive oxygen species (ROS) generated by dual oxidases (Duox) play a role in innate immunity in many organisms. In this study, a 4,735 bp full-length cDNA of the Pacific white shrimp dual oxidase (LvDuox) gene was cloned; the sequence included an open reading frame of 4,497 bp, encoding a protein of 1,498 as with a calculated mass of 173 kDa. Structural analysis revealed that LvDuox contains several domains. Homology analysis revealed that LvDuox exhibits 96.1%, 67.3% and 67.3% sequence identity with Marsupenaeus japonicas, Drosophila melanogaster and Acyrthosiphon pisum Duox, respectively. The mRNA transcripts of LvDuox were detected in all tested tissues. The mRNA expression of LvDuox was significantly induced in the midgut after Vibrio parahaemolyticus E1 (VPE1) stimulation. After the level of H2O2 in the midgut increased, expression of the superoxide dismutase and catalase genes in the midgut increased significantly. These results suggested that the LvDuox gene was upregulated in the midgut after the challenge by VPE1, and antioxidant genes were involved in the regulation of ROS in the shrimp midgut. LvDuox may therefore be a new target for intestinal disease research in the Pacific white shrimp. |
| 资助项目 | Science and Technology Development Fund Project of Shinan District of Qingdao City[2018-4-001-ZH] ; Chinese Academy of Sciences STS Regional Center Project (Fujian Province) |
| WOS研究方向 | Immunology ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000445344500002 |
| 出版者 | INVERTEBRATE SURVIVAL JOURNAL |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/160202]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, L. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Y.,Wang, B. J.,Wang, M. Q.,et al. Molecular characterization of the dual oxidase (LvDuox) gene from the pacific white shrimp Litopenaeus vannamei[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2018,15:316-326. |
| APA | Chen, Y.,Wang, B. J.,Wang, M. Q.,Liu, M.,Jiang, K. Y.,&Wang, L..(2018).Molecular characterization of the dual oxidase (LvDuox) gene from the pacific white shrimp Litopenaeus vannamei.ISJ-INVERTEBRATE SURVIVAL JOURNAL,15,316-326. |
| MLA | Chen, Y.,et al."Molecular characterization of the dual oxidase (LvDuox) gene from the pacific white shrimp Litopenaeus vannamei".ISJ-INVERTEBRATE SURVIVAL JOURNAL 15(2018):316-326. |
入库方式： OAI收割
来源：海洋研究所
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