Biological function of a gC1qR homolog (EcgC1qR) of Exopalaemon carinicauda in defending bacteria challenge
文献类型：期刊论文
| 作者 | Zhang, Jiquan1,2,3; Liu, Yujie1; Li, Yanyan1; Su, Naike1; Zhou, Yaru1; Xiang, Jianhai2,3 ; Sun, Yuying1
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2018-11-01 |
| 卷号 | 82页码:378-385 |
| 关键词 | Exopalaemon carinicauda Globular heads of C1q CRISPR/Cas9 Knock-out |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2018.08.046 |
| 通讯作者 | Sun, Yuying(sunyuying125@163.com) |
| 英文摘要 | The gC1qR is a ubiquitously expressed cell protein that interacts with the globular heads of C1q (gC1q) and many other ligands. In this study, one gC1qR homolog gene was obtained from Exopalaemon carinicauda and named EcgC1qR. The complete nucleotide sequence of EcgC1qR contained a 774 bp open reading frame (ORF) encoding EcgC1qR precursor of 257 amino acids. The deduced amino acid sequence of EcgC1qR revealed a 55- amino-acid-long mitochondrial targeting sequence at the N-terminal and a mitochondrial acidic matrix protein of 33 kDa (MAM33) domain. The genomic organization of EcgC1qR gene showed that EcgC1qR gene contained five exons and four introns. EcgC1qR could express in all of the detected tissues and its expression was much higher in hepatopancreas and hemocytes. The expression of EcgC1qR in the hepatopancreas of prawns challenged with Vibrio parahaemolyticus and Aeromonas hydrophila changed in a time-dependent manner. The expression of EcgC1qR in prawns challenged with V. parahaemolyticus was up-regulated at 6h (p < 0.05), and significantly up-regulated at 12 h and 24 h (p < 0.01), and then returned to the control levels at 48 h post-challenge (p > 0.05). At the same time, the expression in Aeromonas-challenged group was significantly up-regulated at 6, 12 and 24 h. The recombinant EcgC1qR could inhibit the growth of two tested bacteria. In addition, we successfully deleted EcgC1qR gene through CRISPR/Cas9 technology and it was the first time to obtain the mutant of gC1qR homolog gene in crustacean. It's a great progress to study the biological function of gC1qR in crustacean in future. |
| 资助项目 | National Natural Science Foundation of China[31872613] ; National Natural Science Foundation of China[41876196] ; National Natural Science Foundation of China[31772885] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000447571600044 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/160436]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Yuying |
| 作者单位 | 1.Hebei Univ, Coll Life Sci, Baoding 071002, Hebei, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jiquan,Liu, Yujie,Li, Yanyan,et al. Biological function of a gC1qR homolog (EcgC1qR) of Exopalaemon carinicauda in defending bacteria challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2018,82:378-385. |
| APA | Zhang, Jiquan.,Liu, Yujie.,Li, Yanyan.,Su, Naike.,Zhou, Yaru.,...&Sun, Yuying.(2018).Biological function of a gC1qR homolog (EcgC1qR) of Exopalaemon carinicauda in defending bacteria challenge.FISH & SHELLFISH IMMUNOLOGY,82,378-385. |
| MLA | Zhang, Jiquan,et al."Biological function of a gC1qR homolog (EcgC1qR) of Exopalaemon carinicauda in defending bacteria challenge".FISH & SHELLFISH IMMUNOLOGY 82(2018):378-385. |
入库方式： OAI收割
来源：海洋研究所
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