中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Identification and characterization of the psychrophilic bacterium CidnaK gene in the Antarctic Chlamydomoas sp. ICE-L under freezing conditions

文献类型:期刊论文

作者Liu, Chenlin1,2; Zhao, Xia3; Wang, Xiuliang2,4
刊名JOURNAL OF APPLIED PHYCOLOGY
出版日期2018-12-01
卷号30期号:6页码:3519-3528
关键词Antarctic sea-ice Chlamydomonas Chlorophyta DnaK/Hsp70 Horizontal gene transfer Freezing tolerance
ISSN号0921-8971
DOI10.1007/s10811-018-1492-4
通讯作者Liu, Chenlin(ch.lliu@163.com)
英文摘要Heat shock protein DnaK (Hsp70) can prevent irreversible protein denaturation by chaperoning the unfolded polypeptides under extremely cold environment, and enhance cold and freezing tolerance of microorganism. From an Antarctic sea-ice green alga Chlamydomonas sp. ICE-L, eight Hsp70 genes were identified and divided into seven subfamilies: four in the cytoplasmic subfamilies, two in the chloroplast subgroups, and one in the mitochondria or endoplasmic reticulum subfamilies. Phylogenetic analyses showed that except CidnaK, each of the other Cihsp70 genes had their homologs in Chlamydomonas reinhardtii and Volvox carteri. CiDnak is a cytoplasmic protein with highly homologous to DnaK proteins from prokaryotes, and it had 100% amino acid sequence identities with the DnaK of Psychroflexus torquis ATCC 700755, a psychrophilic bacterium isolated from Antarctic sea ice. The transcription of CidnaK was significantly induced upon freezing stress in ICE-L, eminently higher than that of other Cihsp70 genes. In addition, the transformed C. reinhardtii 137c with CidnaK gene showed much higher rates of survival and growth than the wild type under freezing or low temperature conditions. Our results suggested that CidnaK was likely originated from the symbiotic bacterium in the sea-ice brine, and is an important candidate freezing inducible gene that confers low temperature tolerance to Chlamydomonas cells, and plays a crucial role in ICE-L adapting the frigid Antarctic environments.
资助项目National Natural Science Foundation[41276203] ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances
WOS研究方向Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种英语
WOS记录号WOS:000455405900052
出版者SPRINGER
源URL[http://ir.qdio.ac.cn/handle/337002/160617]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Liu, Chenlin
作者单位1.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
3.Qingdao Univ, Coll Environm Sci & Engn, Qingdao 266000, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
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Liu, Chenlin,Zhao, Xia,Wang, Xiuliang. Identification and characterization of the psychrophilic bacterium CidnaK gene in the Antarctic Chlamydomoas sp. ICE-L under freezing conditions[J]. JOURNAL OF APPLIED PHYCOLOGY,2018,30(6):3519-3528.
APA Liu, Chenlin,Zhao, Xia,&Wang, Xiuliang.(2018).Identification and characterization of the psychrophilic bacterium CidnaK gene in the Antarctic Chlamydomoas sp. ICE-L under freezing conditions.JOURNAL OF APPLIED PHYCOLOGY,30(6),3519-3528.
MLA Liu, Chenlin,et al."Identification and characterization of the psychrophilic bacterium CidnaK gene in the Antarctic Chlamydomoas sp. ICE-L under freezing conditions".JOURNAL OF APPLIED PHYCOLOGY 30.6(2018):3519-3528.

入库方式: OAI收割

来源:海洋研究所

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