Design and Synthesis of a Fluorescent Probe with a Large Stokes Shift for Detecting Thiophenols and Its Application in Water Samples and Living Cells
文献类型：期刊论文
| 作者 | Liu, Hua1,2,3,4 ; Guo, Chuanlong1,5; Guo, Shuju1,2,3; Wang, Lijun1,2,3; Shi, Dayong1,2,3,4
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| 刊名 | MOLECULES
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| 出版日期 | 2019-01-02 |
| 卷号 | 24期号:2页码:14 |
| 关键词 | fluorescent probe thiophenol water samples chalcone living cells |
| ISSN号 | 1420-3049 |
| DOI | 10.3390/molecules24020375 |
| 通讯作者 | Wang, Lijun(wanglijun@qdio.ac.cn) ; Shi, Dayong(shidayong@qdio.ac.cn) |
| 英文摘要 | A turn-on florescent probe (probe-KCP) was developed for highly selective detection of thiophenols based on a donor-excited photo-induced electron transfer mechanism. Herein, the synthesis of the probe, a chalcone derivative, through a simple straightforward combination of a carbazole-chalcone fluorophore with a 2,4-dinitrophenyl functional group. In a kinetic study of the probe-KCP for thiophenols, the probe displayed a short response time (30 min) and significant fluorescence enhancement. In selection and competition experiments, the probe-KCP exhibited excellent selectivity for thiophenols over glutathione (GSH), cysteine (Cys), sodium hydrosulfide (NaSH), and ethanethiol (C2H5SH) in addition to common anions and metal ions. Using the designed probe, we successfully monitored and quantified thiophenols, which are highly toxic. This turn-on fluorescence probe features a remarkably large Stokes shift (130 nm) and a short response time (30 min), and it is highly selective and sensitive (160-fold) in the detection of thiophenols, with marked fluorescence in the presence of thiophenols. probe-KCP responds to thiophenols with a good range of linearity (0-15 M) and a detection limit of 28 nM (R-2 = 0.9946) over other tested species mentioned including aliphatic thiols, thiophenol analogues, common anions, and metal ions. The potential applications of this carbazole-chalcone fluorescent probe was successfully used to determine of thiophenols in real water samples and living cells with good performance and low cytotoxicity. Therefore, this probe has great potential application in environment and biological samples. |
| 资助项目 | National Natural Science Foundation of China[81773586] ; National Natural Science Foundation of China[81703354] ; National Natural Science Foundation of China[81600782] ; Shandong Provincial Natural Science Foundation for Distinguished Young Scholars[JQ201722] ; Key Research Program of Frontier Sciences, CAS[QYZDB-SSW-DQC014] ; Project of Discovery, Evaluation and Transformation of Active Natural Com-pounds, Strategic Biological Resources Service Network Program of Chinese Academy of Sciences[ZSTH-026] ; National Program for Support of Top-notch Young Professionals ; Taishan scholar Youth Project of Shandong province ; Key research and development project of Shandong province[2018GSF118223] ; Natural Science Foundation of Shandong Province[ZR2016DQ07] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000457137200161 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/160661]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Lijun; Shi, Dayong |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Qingdao Univ Sci & Technol, Dept Pharm, Coll Chem Engn, Qingdao 266042, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Hua,Guo, Chuanlong,Guo, Shuju,et al. Design and Synthesis of a Fluorescent Probe with a Large Stokes Shift for Detecting Thiophenols and Its Application in Water Samples and Living Cells[J]. MOLECULES,2019,24(2):14. |
| APA | Liu, Hua,Guo, Chuanlong,Guo, Shuju,Wang, Lijun,&Shi, Dayong.(2019).Design and Synthesis of a Fluorescent Probe with a Large Stokes Shift for Detecting Thiophenols and Its Application in Water Samples and Living Cells.MOLECULES,24(2),14. |
| MLA | Liu, Hua,et al."Design and Synthesis of a Fluorescent Probe with a Large Stokes Shift for Detecting Thiophenols and Its Application in Water Samples and Living Cells".MOLECULES 24.2(2019):14. |
入库方式： OAI收割
来源：海洋研究所
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