A preliminary attempt to explore the potential functions of a tetraspanin gene (MmTSPAN) in the innate immunity of hard clam Meretrix meretrix: Sequence features and expression profiles
文献类型：期刊论文
| 作者 | Wang, Yan1,3; Wang, Mengqiang1,4 ; Wang, Baojie1; Liu, Mei1; Jiang, Keyong1; Hou, Xuguang5; Wang, Lei1,2,6
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2019-05-01 |
| 卷号 | 88页码:135-141 |
| 关键词 | Meretrix meretrix Tetraspanin Innate immunity |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2019.01.048 |
| 通讯作者 | Wang, Lei(wanglei@qdio.ac.cn) |
| 英文摘要 | Tetraspanins belong to the transmembrane 4 superfamily (TM4SF), and play crucial roles in immune responses. In the present study, a novel tetraspanin gene (designated MmTSPAN) was cloned and characterized from the hard clam Meretrix meretrix. The complete cDNA sequence of MmTSPAN contained an open reading frame (ORF) of 816 bp, which encoded a protein of 271 amino acids. MmTSPAN exhibited highly similarity with previously identified tetraspanins from other species. It contained four transmembrane domains (12-35 aa, 69-92 aa, 99-123 aa and 238-261 aa), characteristic CCG motif and four conservative cysteine residues. The mRNA transcripts of MmTSPAN were ubiquitously detectable in all the tested tissues, with the highest expression level in hepatopancreas. Temporal transcriptional levels in the hepatopancreas revealed significant up-regulation of MmTSPAN by Vibrio splendidus stimulation, with a 3.14-fold increase at 6h compared to the control, and reaching 32.98-fold at 24 h. These results provide useful information for further study of the function of tetraspanin in the innate immune system of M. meretrix, and may offer a new therapeutic target for diseases of M. meretrix. |
| 资助项目 | Major Special Science and Technology Project of Shandong Province, China[2018SDKJ0] ; Science and Technology Development Fund Project of Shinan district of Qingdao city, China[2018-4-001-ZH] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000465060500016 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/161077]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Lei |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Shandong, Peoples R China 5.Shandong Univ Weihai, Marine Coll, Weihai 264209, Peoples R China 6.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yan,Wang, Mengqiang,Wang, Baojie,et al. A preliminary attempt to explore the potential functions of a tetraspanin gene (MmTSPAN) in the innate immunity of hard clam Meretrix meretrix: Sequence features and expression profiles[J]. FISH & SHELLFISH IMMUNOLOGY,2019,88:135-141. |
| APA | Wang, Yan.,Wang, Mengqiang.,Wang, Baojie.,Liu, Mei.,Jiang, Keyong.,...&Wang, Lei.(2019).A preliminary attempt to explore the potential functions of a tetraspanin gene (MmTSPAN) in the innate immunity of hard clam Meretrix meretrix: Sequence features and expression profiles.FISH & SHELLFISH IMMUNOLOGY,88,135-141. |
| MLA | Wang, Yan,et al."A preliminary attempt to explore the potential functions of a tetraspanin gene (MmTSPAN) in the innate immunity of hard clam Meretrix meretrix: Sequence features and expression profiles".FISH & SHELLFISH IMMUNOLOGY 88(2019):135-141. |
入库方式： OAI收割
来源：海洋研究所
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