Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research
文献类型：期刊论文
| 作者 | Li, Qianqian1 ; Zhang, Litao1,2; Pang, Tong1,2; Liu, Jianguo1,2
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| 刊名 | EUROPEAN JOURNAL OF PHYCOLOGY
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| 出版日期 | 2019-04-03 |
| 卷号 | 54期号:2页码:162-174 |
| 关键词 | Antenna protein extreme temperature Kappaphycus alvarezii photosynthesis thylakoid membrane proteins transcriptome |
| ISSN号 | 0967-0262 |
| DOI | 10.1080/09670262.2018.1536283 |
| 通讯作者 | Liu, Jianguo(jgliu@qdio.ac.cn) |
| 英文摘要 | Kappaphycus alvarezii provides an important raw material for the extraction of kappa-carrageenan. Comparative transcriptome analysis of K. alvarezii after 24 h extreme temperature treatments (17 degrees C and 34 degrees C) was performed to uncover the molecular mechanisms controlling temperature-related photosynthesis regulation. Optimum temperature (28 degrees C) was employed as the control. After de novo assembly of these three transcriptome samples and searching the assembled transcripts by BLAST against the NCBI nr databases, 11,537 top-hit transcripts were assigned as unigenes. Through comparisons, 302 significantly differentially expressed genes were identified. Twenty-three of the differentially expressed unigenes encoded photosystem antenna protein subunits and a further 25 were associated more broadly with photosynthesis functions. Compared to the light-harvesting complex (LHC) protein, the expression of phycobiliproteins (PBPs) was stable after extreme low and high temperature (ELT/EHT) treatments. Both ELT and EHT were found to inhibit the expression of subunits of photosystem II (PsbA, PsbC, Psb27, PsbW, PsbS), photosystem I (PsaF, PsaG, PsaK) and photosynthetic electron transport (PetE). These results were confirmed through targeted analysis of the mRNA and protein abundance of individual proteins, and were congruent with the results of chlorophyll a fluorescence (OJIP) measurements performed with temperature-stressed lines. In conclusion, similarities and differences in the behaviours of photosynthesis-related genes were summarized to provide opportunities for understanding the responses of K. alvarezii after extreme temperature treatments. |
| 资助项目 | National Natural Science Foundation of China[31702366] ; National Natural Science Foundation of China[41306154] ; Open Foundation of Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences[LMB17011007] |
| WOS研究方向 | Plant Sciences ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000463785700003 |
| 出版者 | TAYLOR & FRANCIS LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/161092]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Jianguo |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Qianqian,Zhang, Litao,Pang, Tong,et al. Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research[J]. EUROPEAN JOURNAL OF PHYCOLOGY,2019,54(2):162-174. |
| APA | Li, Qianqian,Zhang, Litao,Pang, Tong,&Liu, Jianguo.(2019).Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research.EUROPEAN JOURNAL OF PHYCOLOGY,54(2),162-174. |
| MLA | Li, Qianqian,et al."Comparative transcriptome profiling of Kappaphycus alvarezii (Rhodophyta, Gigartinales) in response to two extreme temperature treatments: an RNA-seq-based resource for photosynthesis research".EUROPEAN JOURNAL OF PHYCOLOGY 54.2(2019):162-174. |
入库方式： OAI收割
来源：海洋研究所
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