pol-miR-194a of Japanese flounder (Paralichthys olivaceus) suppresses type I interferon response and facilitates Edwardsiella tarda infection
文献类型：期刊论文
| 作者 | Guan, Xiao-lu1,2,3; Zhang, Bao-cun4,5; Sun, Li1,2 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2019-04-01 |
| 卷号 | 87页码:220-225 |
| 关键词 | miRNA Paralichthys olivaceus Edwardsiella tarda Type I interferon Immune escape |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2019.01.017 |
| 通讯作者 | Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | MicroRNAs (miRNAs) are a type of small non-coding RNAs that participate in diverse cellular processes including microbial invasion and immune defense. In a previous study, we identified a large amount of Japanese flounder (Paralichthys olivaceus) miRNAs responsive to megalocytivirus infection. In the present study, we examined the function of one of these miRNAs, pol-miR-194a, in association with the infectivity of Edwardsiella tarda, an intracellular bacterial pathogen to many fish species including flounder. We found that pol-miR-194a was induced in expression to a significant extent in the spleen, liver, and gill of Japanese flounder infected by E. tarda. Transfection of flounder cells with pol-miR-194a mimic significantly enhanced the intracellular replication of E. tarda. pol-miR-194a was able to interact specifically with the 3'UTR of IRF7 in a negative manner, resulting in inhibition of IRF7 expression. Consistently, pol-miR-194a significantly blocked the promoter activity of type I interferon. Taken together, these results indicate that pol-miR-194a plays an important role in the regulation of flounder immune response as well as microbial infection, and that pol-miR-194a probably serves as a target for E. tarda to manipulate and escape host immune defense. |
| 资助项目 | National Natural Science Foundation of China[31730100] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; National Key R&D Program of China[2018YFD0900504] ; AoShan Talents Program ; Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000464086600025 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/161113]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, CAS Ctr Ocean Mega Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Aarhus Univ, Dept Biomed, Aarhus, Denmark 5.Aarhus Univ, Aarhus Res Ctr Innate Immun, Aarhus, Denmark |
| 推荐引用方式 GB/T 7714 | Guan, Xiao-lu,Zhang, Bao-cun,Sun, Li. pol-miR-194a of Japanese flounder (Paralichthys olivaceus) suppresses type I interferon response and facilitates Edwardsiella tarda infection[J]. FISH & SHELLFISH IMMUNOLOGY,2019,87:220-225. |
| APA | Guan, Xiao-lu,Zhang, Bao-cun,&Sun, Li.(2019).pol-miR-194a of Japanese flounder (Paralichthys olivaceus) suppresses type I interferon response and facilitates Edwardsiella tarda infection.FISH & SHELLFISH IMMUNOLOGY,87,220-225. |
| MLA | Guan, Xiao-lu,et al."pol-miR-194a of Japanese flounder (Paralichthys olivaceus) suppresses type I interferon response and facilitates Edwardsiella tarda infection".FISH & SHELLFISH IMMUNOLOGY 87(2019):220-225. |
入库方式： OAI收割
来源：海洋研究所
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