The first identification of a malectin gene (CfMal) in scallop Chlamys farreri: sequence features and expression profiles
文献类型：期刊论文
| 作者 | Wang, M. Q.1,3; Wang, B. J.1; Liu, M.1; Jiang, K. Y.1; Wang, L.1,2,4
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| 刊名 | ISJ-INVERTEBRATE SURVIVAL JOURNAL
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| 出版日期 | 2019 |
| 卷号 | 16页码:25-33 |
| 关键词 | Chlamys farreri innate immunity malectin |
| ISSN号 | 1824-307X |
| 通讯作者 | Wang, L.(wanglei@qdio.ac.cn) |
| 英文摘要 | Malectin is a newly discovered lectin of the endoplasmic reticulum (ER) that might be involved in innate immunity. Information about the roles of malectin in innate immunity is scarce. In the present study, a novel malectin gene (designated as CfMal) from the Zhikong scallop Chlamys farreri was identified and characterized. Sequence features, tissue distribution, and temporal expression profiles were investigated to infer the potential functions of CfMal in innate immunity. The complete cDNA sequence of CfMal comprised 1,111 bp and contained an open reading frame of 909 bp, which encoded 302 amino acid residues. A malectin domain and a transmembrane region were identified in the predicted protein sequence. CfMal mRNA transcripts were detectable in hemocytes, muscle, mantle, gill, hepatopancreas, and gonads. CfMal expression was highest in hemocytes. Stimulation with Vibrio splendidus increased CfMal expression in hemocytes, gill, and hepatopancreas. The mRNA transcripts of CfMal and three related genes, including binding immunoglobulin protein, heat shock protein 90 kDa beta member 1 protein and ER degradation enhancing alpha-mannosidase like protein 1, increased in scallop hemocytes during an artificial ER-stress. Our results indicate that CfMal might not only be involved in ER-stress, but may also play a role in innate immunity of scallops. |
| 资助项目 | National Natural Science Foundation of China[U1706209] ; Aoshan Innovation Project in Science and Technology from National Laboratory for Marine Science and Technology[2016ASKJ07] ; Science and Technology Service Network Plan (STS) Major Deployment Project[KFZD-SW-106] ; STS Regional Centre Project (Fujian) from the Chinese Academy of Sciences |
| WOS研究方向 | Immunology ; Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000466031500002 |
| 出版者 | INVERTEBRATE SURVIVAL JOURNAL |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/161361]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, L. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Shandong, Peoples R China 4.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Qingdao 266400, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, M. Q.,Wang, B. J.,Liu, M.,et al. The first identification of a malectin gene (CfMal) in scallop Chlamys farreri: sequence features and expression profiles[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2019,16:25-33. |
| APA | Wang, M. Q.,Wang, B. J.,Liu, M.,Jiang, K. Y.,&Wang, L..(2019).The first identification of a malectin gene (CfMal) in scallop Chlamys farreri: sequence features and expression profiles.ISJ-INVERTEBRATE SURVIVAL JOURNAL,16,25-33. |
| MLA | Wang, M. Q.,et al."The first identification of a malectin gene (CfMal) in scallop Chlamys farreri: sequence features and expression profiles".ISJ-INVERTEBRATE SURVIVAL JOURNAL 16(2019):25-33. |
入库方式： OAI收割
来源：海洋研究所
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