Investigation of the Amide Proton Solvent Exchange Properties of Glycosaminoglycan Oligosaccharides
文献类型：期刊论文
| 作者 | Green, Andrew R.1; Li, Kecheng1,2,3; Lockard, Blake1; Young, Robert P.1,4; Mueller, Leonard J.1; Larive, Cynthia K.1 |
| 刊名 | JOURNAL OF PHYSICAL CHEMISTRY B
 |
| 出版日期 | 2019-06-06 |
| 卷号 | 123期号:22页码:4653-4662 |
| ISSN号 | 1520-6106 |
| DOI | 10.1021/acs.jpcb.9b01794 |
| 通讯作者 | Larive, Cynthia K.(clarive@ucr.edu) |
| 英文摘要 | One-dimensional H-1 NMR experiments were conducted for aqueous solutions of glycosaminoglycan oligosaccharides to measure the amide proton temperature coefficients and activation energy barriers for solvent exchange and evaluate the effect of pH on the solvent exchange properties. A library of mono- and oligosaccharides was prepared by enzymatic depolymerization of amide-containing polysaccharides and by chemical modification of heparin and heparan sulfate saccharides including members that contain a 3-O-sulfated glucosamine residue. The systematic evaluation of this saccharide library facilitated assessment of the effects of structural characteristics, such as size, sulfation number and site, and glycosidic linkage, on amide proton solvent exchange rates. Charge repulsion by neighboring negatively charged sulfate and carboxylate groups was found to have a significant impact on the catalysis of amide proton solvent exchange by hydroxide. This observation leads to the conclusion that solvent exchange rates must be interpreted within the context of a given chemical environment. On their own, slow exchange rates do not conclusively establish the involvement of a labile proton in a hydrogen bond, and additional supporting experimental evidence such as reduced temperature coefficients is required. |
| 资助项目 | NSF[CHE-1626673] ; NIH[R01GM097569] ; China Scholarship Council[201504910405] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000470938100005 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/161834]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Larive, Cynthia K. |
| 作者单位 | 1.Univ Calif Riverside, Dept Chem, Riverside, CA 92501 USA 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Shandong, Peoples R China 4.Pacific Northwest Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA |
| 推荐引用方式 GB/T 7714 | Green, Andrew R.,Li, Kecheng,Lockard, Blake,et al. Investigation of the Amide Proton Solvent Exchange Properties of Glycosaminoglycan Oligosaccharides[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2019,123(22):4653-4662. |
| APA | Green, Andrew R.,Li, Kecheng,Lockard, Blake,Young, Robert P.,Mueller, Leonard J.,&Larive, Cynthia K..(2019).Investigation of the Amide Proton Solvent Exchange Properties of Glycosaminoglycan Oligosaccharides.JOURNAL OF PHYSICAL CHEMISTRY B,123(22),4653-4662. |
| MLA | Green, Andrew R.,et al."Investigation of the Amide Proton Solvent Exchange Properties of Glycosaminoglycan Oligosaccharides".JOURNAL OF PHYSICAL CHEMISTRY B 123.22(2019):4653-4662. |
入库方式： OAI收割
来源：海洋研究所
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