DNA-templated fluorescent silver nanoclusters for sensitive detection of pathogenic bacteria based on MNP-DNAzyme-AChE complex
文献类型:期刊论文
作者 | Zheng, Laibao1,2,3,4; Qi, Peng1,2,4; Zhang, Dun1,2,4 |
刊名 | SENSORS AND ACTUATORS B-CHEMICAL |
出版日期 | 2018-12-10 |
卷号 | 276页码:42-47 |
ISSN号 | 0925-4005 |
关键词 | DNA-template silver nanocluster DNAzyme DNA-enzyme conjugate Bacterial detection |
DOI | 10.1016/j.snb.2018.08.078 |
通讯作者 | Qi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn) |
英文摘要 | The rapid and sensitive detection of bacteria is of great importance for the field of food safety, water quality and clinical diagnosis. Herein, we present a novel DNA-templated fluorescent silver nanoclusters (DNA-AgNCs) based sensing system integrated with magnetic nanoparticles (MNP)-DNAzyme-AChE complex for detection of pathogenic bacterial using Escherichia coli (E. coli) as a target bacteria. The platform takes advantage of a DNA-AgNCs based enzyme-responsive fluorescent signal element and a DNAzyme recognition element, providing an ultrasensitive detection limit of 60 cfu mL(-1), and good specificity to the target bacteria. Moreover, the platform could be developed into a potentially versatile system by simply altering corresponding DNAzyme as the objects recognition probes. |
资助项目 | National Natural Science Foundation of China[41506094] ; National Key Basic Research Program of China[2014CB643304] ; Science & Technology Basic Research Program of Qingdao[16-5-1-19-jch] ; Qingdao National Laboratory for Marine Science and Technology[017ASTCP-ES02] |
WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE SA |
WOS记录号 | WOS:000444637600006 |
源URL | [http://ir.qdio.ac.cn/handle/337002/160205] |
专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
通讯作者 | Qi, Peng; Zhang, Dun |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Zheng, Laibao,Qi, Peng,Zhang, Dun. DNA-templated fluorescent silver nanoclusters for sensitive detection of pathogenic bacteria based on MNP-DNAzyme-AChE complex[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,276:42-47. |
APA | Zheng, Laibao,Qi, Peng,&Zhang, Dun.(2018).DNA-templated fluorescent silver nanoclusters for sensitive detection of pathogenic bacteria based on MNP-DNAzyme-AChE complex.SENSORS AND ACTUATORS B-CHEMICAL,276,42-47. |
MLA | Zheng, Laibao,et al."DNA-templated fluorescent silver nanoclusters for sensitive detection of pathogenic bacteria based on MNP-DNAzyme-AChE complex".SENSORS AND ACTUATORS B-CHEMICAL 276(2018):42-47. |
入库方式: OAI收割
来源:海洋研究所
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