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Staphylococcal enterotoxin C2 stimulated the maturation of bone marrow derived dendritic cells via TLR-NF kappa B signaling pathway

文献类型：期刊论文

作者Liao, Hui3; Yao, Songyuan1,2; Xu, Mingkai1; Li, Yansheng1,2; Zhou, Libao3; Zhang, Huiwen1; Zhang, Chenggang1
刊名EXPERIMENTAL CELL RESEARCH
出版日期2018-09-15
卷号370期号:2页码:237-244
关键词Bone Marrow-derived Dendritic Cells Nf Kappa b Staphylococcal Enterotoxin C2 Toll Like Receptors
ISSN号0014-4827
DOI10.1016/j.yexcr.2018.06.024
英文摘要As a kind of superantigen, staphylococcal enterotoxin C2 (SEC2) is well known as a powerful immunomodulator. However, most previous studies about SEC2 focus on its T cell activating characters. But the direct effect of SEC2 on antigen-presenting cells (APCs) which are important for the T cell activation is not clearly. In this study, we investigated the effect of SEC2 on murine bone marrow-derived dendritic cells (BMDCs) which are known as the specialized professional APCs. Contrary to its effects on T cells, SEC2 could not induce proliferation or cytotoxicity to BMDCs even in high concentrations. While SEC2 could promote the mature of BMDCs with increased expression of co-stimulatory molecules on cell membrane such as CD80, CD86, and MHC II. The production of pro-inflammatory cytokines such as TNF-alpha, IFN-gamma and IL-6 were also increased in BMDCs treated with SEC2. We also found that SEC2 enhanced the genes expression of pattern recognition receptors including toll-like receptors 2 (TLR2) and TLR4 in BMDCs, and up-regulated the key signal molecule MyD88 in both mRNA and protein levels. In addition, SEC2 also caused I kappa B alpha degradating and NF kappa B p65 translocating from the cytoplasm to the nucleus in BMDCs. The siRNAs for both TLR2 and TLR4, as well as NFKB specific inhibitor BAY 11-7085 could inhibit the co-stimulatory molecules expression and pro-inflammatory cytokines releasing induced by SEC2. Moreover, TLR2/4 specific siRNAs inhibited p65 and MyD88 upregulation induced by SEC2. In summary, all our results indicated that SEC2 could stimulate BMDCs maturation through TLR-NFKB signaling pathways.
WOS研究方向Oncology ; Cell Biology
语种英语
WOS记录号WOS:000442979100006
出版者ELSEVIER INC
源URL[http://210.72.129.5/handle/321005/121239]  
专题中国科学院沈阳应用生态研究所
通讯作者Xu, Mingkai
作者单位1.Chinese Acad Sci, Inst Appl Ecol, Shenyang, Liaoning, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chengda Biotechnol Co Ltd, Shenyang, Liaoning, Peoples R China
推荐引用方式
GB/T 7714		 Liao, Hui,Yao, Songyuan,Xu, Mingkai,et al. Staphylococcal enterotoxin C2 stimulated the maturation of bone marrow derived dendritic cells via TLR-NF kappa B signaling pathway[J]. EXPERIMENTAL CELL RESEARCH,2018,370(2):237-244.
APA Liao, Hui.,Yao, Songyuan.,Xu, Mingkai.,Li, Yansheng.,Zhou, Libao.,...&Zhang, Chenggang.(2018).Staphylococcal enterotoxin C2 stimulated the maturation of bone marrow derived dendritic cells via TLR-NF kappa B signaling pathway.EXPERIMENTAL CELL RESEARCH,370(2),237-244.
MLA Liao, Hui,et al."Staphylococcal enterotoxin C2 stimulated the maturation of bone marrow derived dendritic cells via TLR-NF kappa B signaling pathway".EXPERIMENTAL CELL RESEARCH 370.2(2018):237-244.

入库方式： OAI收割

来源：沈阳应用生态研究所

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