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Oxidative stress and DNA damage in the earthworm Eisenia fetida induced by toluene, ethylbenzene and xylene

文献类型：期刊论文

作者Liu, Yao2; Zhou, Qixing1,2; Xie, Xiujie2; Lin, Dasong2; Dong, Luxi2
刊名ECOTOXICOLOGY
出版日期2010-11-01
卷号19期号:8页码:1551-1559
关键词Toluene Ethylbenzene Xylene Oxidative Stress Genotoxicity Earthworm (Eisenia Fetida) Comet Assay (Scge)
ISSN号0963-9292
DOI10.1007/s10646-010-0540-x
英文摘要Superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT), and the comet assay (SCGE) were used as biomarkers to evaluate the oxidative stress and genotoxicity of toluene, ethylbenzene and xylene in earthworms (Eisenia fetida). The results indicated that the exposure of the three pollutants caused a stress response of the three enzymes, an approximate bell-shaped change (a tendency of inducement firstly and then inhibition with increasing concentrations of the pollutants) was mostly found. The three enzymes tested differed in their sensitivity to different pollutants. While the activity of POD was not significantly changed within the concentration range, the concentration thresholds for significant (P < 0.05) responses to toluene based on SOD and CAT were 5 mg kg(-1), respectively. Similarly, the concentration thresholds for significant (P < 0.05) responses to ethylbenzene based on CAT and POD were 10 and 5 mg kg(-1), respectively, while the activity of SOD was not significantly changed within the concentration range. Significant responses to xylene based on CAT and POD were 5 mg kg(-1), respectively, while the activity of SOD was significantly (P < 0.05) induced at 10 mg kg(-1). The SCGE assay results showed that these three pollutants could significantly (P < 0.01) induce DNA damage in earthworms and the clear dose-dependent relationships were displayed, indicating potential genotoxic effects of toluene, ethylbenzene, and xylene on E. fetida. The inducement of DNA damage may be attributed to the oxidative attack of toluene, ethylbenzene, and xylene. Toluene seemed to be more genotoxic as it could induce the higher extent of DNA damage than ethylbenzene and xylene. The results suggest that the SCGE assay of earthworms is simple and efficient for diagnosing the genotoxicity of pollutants in terrestrial environment.
WOS研究方向Environmental Sciences & Ecology ; Toxicology
语种英语
WOS记录号WOS:000284363800018
出版者SPRINGER
源URL[http://210.72.129.5/handle/321005/121407]  
专题中国科学院沈阳应用生态研究所
通讯作者Zhou, Qixing
作者单位1.Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
2.Nankai Univ, Coll Environm Sci & Engn, Minist Educ, Key Lab Pollut Proc & Environm Criteria, Tianjin 300071, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Yao,Zhou, Qixing,Xie, Xiujie,et al. Oxidative stress and DNA damage in the earthworm Eisenia fetida induced by toluene, ethylbenzene and xylene[J]. ECOTOXICOLOGY,2010,19(8):1551-1559.
APA Liu, Yao,Zhou, Qixing,Xie, Xiujie,Lin, Dasong,&Dong, Luxi.(2010).Oxidative stress and DNA damage in the earthworm Eisenia fetida induced by toluene, ethylbenzene and xylene.ECOTOXICOLOGY,19(8),1551-1559.
MLA Liu, Yao,et al."Oxidative stress and DNA damage in the earthworm Eisenia fetida induced by toluene, ethylbenzene and xylene".ECOTOXICOLOGY 19.8(2010):1551-1559.

入库方式： OAI收割

来源：沈阳应用生态研究所

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