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Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement

文献类型：期刊论文

作者Zhang Li-li1,2; Wu Zhi-jie2; Song Yu-chao3
刊名SPECTROSCOPY AND SPECTRAL ANALYSIS
出版日期2010-07-01
卷号30期号:7页码:2011-2013
关键词(15)n Mass Spectrometery Denitrifying Enzyme Activity 3 4-dimethylpyrazole Phosphate
ISSN号1000-0593
DOI10.3964/j.issn.1000-0593(2010)07-2011-03
英文摘要Nitrification inhibitors exert inhibition function in soil nitrification process (NF(4)(+)-N to NO(3)(-)-N) and are widely applied in order to improve N fertilizer use efficiency. Before the new nitrification inhibitor is used, its effects on denitrification process must be investigated and denitrifying enzyme activity (DEA) is an effective indicator to show this process. In the present paper, a mass spectrometery(MS) method was taken to measure the denitrifying enzyme activity in the new nitrification inhibitor 3, 4-dimethylpyrazole phosphate(DMPP) incubation system. The results showed that the method measured the concentration of N(2)O in the incubation system accurately and the concentration measured by MS had marked correlation with that measured by gas chromatogram(GC) (MS(N2O) = -0.45 + 1.03GC(N2O), R(2) = 0.995). At the same time, enrichment of (15)N(2)O and (15)N(2) was measured to discriminate the nitrate reductase and N(2)O reductase. Differed with traditional method, the enzymatic inhibitor-acetylene was not appended. The results showed that DMPP had no influence on the denitrifying enzyme activity and indicated that the denitrification process was not influenced by DMPP.
WOS研究方向Spectroscopy
语种英语
WOS记录号WOS:000279504400064
出版者OFFICE SPECTROSCOPY & SPECTRAL ANALYSIS
源URL[http://210.72.129.5/handle/321005/121461]  
专题中国科学院沈阳应用生态研究所
通讯作者Wu Zhi-jie
作者单位1.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
3.Shenyang Agr Univ, Soil & Environm Coll, Shenyang 110161, Peoples R China
推荐引用方式
GB/T 7714		 Zhang Li-li,Wu Zhi-jie,Song Yu-chao. Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2010,30(7):2011-2013.
APA Zhang Li-li,Wu Zhi-jie,&Song Yu-chao.(2010).Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement.SPECTROSCOPY AND SPECTRAL ANALYSIS,30(7),2011-2013.
MLA Zhang Li-li,et al."Mass Spectrometery Assay for Denitrifying Enzyme Activity Measurement".SPECTROSCOPY AND SPECTRAL ANALYSIS 30.7(2010):2011-2013.

入库方式： OAI收割

来源：沈阳应用生态研究所

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