A PCR-after-ligation method for cloning of multiple DNA inserts
文献类型：期刊论文
| 作者 | An, Yingfeng; Wu, Wenfang; Lv, Anguo |
| 刊名 | ANALYTICAL BIOCHEMISTRY
 |
| 出版日期 | 2010-07-15 |
| 卷号 | 402期号:2页码:203-205 |
| ISSN号 | 0003-2697 |
| DOI | 10.1016/j.ab.2010.03.040 |
| 英文摘要 | Here we present a novel and simple PCR-after-ligation method for efficient assembly of multiple DNA inserts. After initial ligation of multiple inserts and vector, the ligation mixture is used as template for a PCR using a pair of primers flanking the cloning sites on the vector. The fragment with correct size is gel purified and inserted into the vector by conventional two-way ligation. With this method, a recombinant plasmid containing four DNA inserts was correctly constructed. As a control, all of the constructs obtained directly from DNA ligation were found to be self-ligation of the vector. (C) 2010 Elsevier Inc. All rights reserved. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000278423900015 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://210.72.129.5/handle/321005/121499]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Wu, Wenfang |
| 作者单位 | Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Yingfeng,Wu, Wenfang,Lv, Anguo. A PCR-after-ligation method for cloning of multiple DNA inserts[J]. ANALYTICAL BIOCHEMISTRY,2010,402(2):203-205. |
| APA | An, Yingfeng,Wu, Wenfang,&Lv, Anguo.(2010).A PCR-after-ligation method for cloning of multiple DNA inserts.ANALYTICAL BIOCHEMISTRY,402(2),203-205. |
| MLA | An, Yingfeng,et al."A PCR-after-ligation method for cloning of multiple DNA inserts".ANALYTICAL BIOCHEMISTRY 402.2(2010):203-205. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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