A convenient colony-based transformation method for high-throughput changing of plasmid hosts
文献类型：期刊论文
| 作者 | An, Yingfeng; Lv, Anguo; Wu, Wenfang |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 32期号:11页码:1693-1697 |
| 关键词 | Chemical Transformation Electroporation Genetic Transformation High-throughput |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-010-0339-0 |
| 英文摘要 | We have developed a convenient Escherichia coli transformation method, termed colony-based transformation, in which a single fresh colony of plasmid-containing donor strain is used instead of extracted plasmid to transform E. coli recipient cell. Thus the need for plasmid extraction and competent cell preparation is avoided. Additionally, a high-throughput transformation process based on this method was designed in which samples are prepared in a 96-well PCR plate and hundreds of transformations can be performed simultaneously in a thermocycler. We therefore suggest that this method may serve as a substitute of current transformation methods based on plasmid extraction. |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000282696100019 |
| 出版者 | SPRINGER |
| 源URL | [http://210.72.129.5/handle/321005/121551]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Wu, Wenfang |
| 作者单位 | Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Yingfeng,Lv, Anguo,Wu, Wenfang. A convenient colony-based transformation method for high-throughput changing of plasmid hosts[J]. BIOTECHNOLOGY LETTERS,2010,32(11):1693-1697. |
| APA | An, Yingfeng,Lv, Anguo,&Wu, Wenfang.(2010).A convenient colony-based transformation method for high-throughput changing of plasmid hosts.BIOTECHNOLOGY LETTERS,32(11),1693-1697. |
| MLA | An, Yingfeng,et al."A convenient colony-based transformation method for high-throughput changing of plasmid hosts".BIOTECHNOLOGY LETTERS 32.11(2010):1693-1697. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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