Involvements of S-nitrosylation and denitrosylation in the production of polyphenols by Inonotus obliquus
文献类型：期刊论文
| 作者 | Zheng, Weifa4; Liu, Yubing4; Pan, Shenyuan2; Yuan, Weihua4; Dai, Yucheng3; Wei, Jiangchun1 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2011-06-01 |
| 卷号 | 90期号:5页码:1763-1772 |
| 关键词 | Inonotus Obliquus Nitric Oxide S-nitrosylation Denitrosylation Polyphenols |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-011-3241-3 |
| 英文摘要 | Nitric oxide (NO) has been evidenced to mediate biosynthesis of polyphenols in Inonotus obliquus. However, it remains unknown how NO regulates their biosynthesis. Here we show that higher cellular NO levels coincided with higher accumulation of S-nitrosothiols (SNO; the products of NO combined with a specific residue in glutathione or proteins) and polyphenols, and higher activity of denitrosylated S-nitrosoglutathione reductase (GSNOR) and thioredoxin reductase (TrxR). This homeostasis was breached by GSNOR or TrxR inhibitors. Inhibiting GSNOR boosted TrxR activity, but reduced SNO formation, coinciding with an enhanced production of polyphenols. Likewise, inhibiting TrxR increased GSNOR activity and SNO production, but downregulated accumulation of polyphenols. Inhibiting GSNOR or TrxR also modified the polyphenolic profiles of I. obliquus. Suppressing GSNOR-enhanced biosynthesis of phelligridins C and H, inoscavin C and methyl inoscavin B, but reduced that of phelligridin D, methyl inoscavin A, davallialactone and methyl davallialactone, the typical polyphenols in I. obliquus. Similarly, downregulating TrxR increased production of phelligridin D, methyl inoscavin A, davallialactone, and methyl davallialactone, but shrinking that of phelligridins C and H, methyl inoscavin B and inoscavin C. Thus, in I. obliquus, the state of S-nitrosylation and denitrosylation affects not only the accumulation of polyphenols, but also their metabolic profiles. |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000290580400015 |
| 出版者 | SPRINGER |
| 源URL | [http://210.72.129.5/handle/321005/121697]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Zheng, Weifa |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Beijing 110101, Peoples R China 2.Xuzhou Normal Univ, Sch Life Sci, Xuzhou 221116, Peoples R China 3.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110116, Peoples R China 4.Xuzhou Normal Univ, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Weifa,Liu, Yubing,Pan, Shenyuan,et al. Involvements of S-nitrosylation and denitrosylation in the production of polyphenols by Inonotus obliquus[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2011,90(5):1763-1772. |
| APA | Zheng, Weifa,Liu, Yubing,Pan, Shenyuan,Yuan, Weihua,Dai, Yucheng,&Wei, Jiangchun.(2011).Involvements of S-nitrosylation and denitrosylation in the production of polyphenols by Inonotus obliquus.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,90(5),1763-1772. |
| MLA | Zheng, Weifa,et al."Involvements of S-nitrosylation and denitrosylation in the production of polyphenols by Inonotus obliquus".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 90.5(2011):1763-1772. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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