Dynamics of bacterial community structure in a full-scale wastewater treatment plant with anoxic-oxic configuration using 16S rDNA PCR-DGGE fingerprints
文献类型：期刊论文
| 作者 | Ding, Lingling1,3; Zhou, Qixing1,2; Wang, Lin1,3; Zhang, Qianru1 |
| 刊名 | AFRICAN JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2011-01-24 |
| 卷号 | 10期号:4页码:589-600 |
| 关键词 | Community Structure Pcr-dgge 16s Ribosomal Dna Wastewater Treatment Plant (Wwtp) Activated Sludge |
| ISSN号 | 1684-5315 |
| 英文摘要 | The microbial community composition and dominant bacterial populations in anoxic-oxic activated sludge from a full-scale wastewater treatment plant (WWTP), were investigated with polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) coupled with sequence analysis of 16S rRNA gene fragments from dominant bands. The bacterial ecological distribution at different parts of each reactor, the dynamics of the bacterial community structure over the total process course and temporal dynamics were obtained by comparing the DGGE patterns. Differences were noted between three sections of the reactors and also two reactors within the entire process of the system. Temporal variations in bacterial composition of the activated sludge in each reactor were responded to their circumstances. The prominent DGGE bands were excised and sequenced in order to identify the predominantly present and active bacterial populations. Here, combined with phylogenetic analysis of the DGGE fingerprints, the four major bacterial lineages can be revealed: alpha, beta, y- Proteobacteria, and the phylum Firmicutes (low G+C Gram-positive). The 16S rDNA sequence analysis indicated that the DGGE bands of dominant bacterial from this plant harbored sequences of possible nitrogen remover with potential aerobic denitrification / heterotrophic nitrification. |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000286526500018 |
| 出版者 | ACADEMIC JOURNALS |
| 源URL | [http://210.72.129.5/handle/321005/121709]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Zhou, Qixing |
| 作者单位 | 1.Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China 2.Nankai Univ, Coll Environm Sci & Engn, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Lingling,Zhou, Qixing,Wang, Lin,et al. Dynamics of bacterial community structure in a full-scale wastewater treatment plant with anoxic-oxic configuration using 16S rDNA PCR-DGGE fingerprints[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(4):589-600. |
| APA | Ding, Lingling,Zhou, Qixing,Wang, Lin,&Zhang, Qianru.(2011).Dynamics of bacterial community structure in a full-scale wastewater treatment plant with anoxic-oxic configuration using 16S rDNA PCR-DGGE fingerprints.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(4),589-600. |
| MLA | Ding, Lingling,et al."Dynamics of bacterial community structure in a full-scale wastewater treatment plant with anoxic-oxic configuration using 16S rDNA PCR-DGGE fingerprints".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.4(2011):589-600. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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