Temporal responses of soil microorganisms to substrate addition as indicated by amino sugar differentiation
文献类型：期刊论文
| 作者 | He, Hongbo2; Zhang, Wei2; Zhang, Xudong2,3; Xie, Hongtu2; Zhuang, Jie1,2 |
| 刊名 | SOIL BIOLOGY & BIOCHEMISTRY
 |
| 出版日期 | 2011-06-01 |
| 卷号 | 43期号:6页码:1155-1161 |
| 关键词 | Isotope Differentiation Amino Sugar Temporal Response Microbial Community (15)n Incubation Glucose |
| ISSN号 | 0038-0717 |
| DOI | 10.1016/j.soilbio.2011.02.002 |
| 英文摘要 | Amino sugars are one of the important microbial residue biomarkers which are associated with soil organic matter cycling. However, little is known about their transformation kinetics in response to available substrates because living biomass only contributes a negligible portion to the total mass of amino sugars. By using (15)N tracing technique, the newly synthesized (labeled) amino sugars can be differentiated from the native portions in soil matrix, making it possible to evaluate, in quantitative manner, the transformation pattern of amino sugars and to interpret the past and ongoing changes of microbial communities during the assimilation of extraneous (15)N. In this study, laboratory incubations of soil samples were conducted by using (15)NH(4)(+) as nitrogen source with or without glucose addition. Both the (15)N enrichment (expressed as atom percentage excess, APE) and the contents of amino sugars were determined by an isotope-based gas chromatography mass spectrometry. The significant (15)N incorporation into amino sugars was only observed in glucose plus (15)NH(4)(+) amendment with the APE arranged as: muramic acid (MurN) > glucosamine (GlcN) > galactosamine (GalN). The dynamics of (15)N enrichment in bacterial-derived MurN and fungal-derived GlcN were fitted to the hyperbolic equations and indicative for the temporal responses of different soil microorganisms. The APE plateau of MurN and fungal-derived GlcN represented the maximal extent of bacterial and fungal populations, respectively, becoming active in response to the available substrates. The different dynamics of the (15)N enrichment between MurN and GlcN indicated that bacteria reacted faster than fungi to assimilate the labile substrates initially, but fungus growth was dominant afterward, leading to integrated microbial community structure over time. Furthermore, the dynamics of labeled and unlabeled portions of amino sugars were compound-specific and substrate-dependent, suggesting their different stability in soil. GlcN tended to accumulate in soil while MurN was more likely degraded as a carbon source when nitrogen supply was excessive. (C) 2011 Elsevier Ltd. All rights reserved. |
| WOS研究方向 | Agriculture |
| 语种 | 英语 |
| WOS记录号 | WOS:000290698100006 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://210.72.129.5/handle/321005/121725]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Zhang, Xudong |
| 作者单位 | 1.Univ Tennessee, Inst Secure & Sustainable Environm, Dept Biosyst Engn & Soil Sci, Ctr Environm Biotechnol, Knoxville, TN 37996 USA 2.Chinese Acad Sci, Key Lab Terr Ecol Proc, Inst Appl Ecol, Shenyang 110016, Peoples R China 3.Chinese Acad Sci, Natl Field Res Stn Shenyang Agroecosyst, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Hongbo,Zhang, Wei,Zhang, Xudong,et al. Temporal responses of soil microorganisms to substrate addition as indicated by amino sugar differentiation[J]. SOIL BIOLOGY & BIOCHEMISTRY,2011,43(6):1155-1161. |
| APA | He, Hongbo,Zhang, Wei,Zhang, Xudong,Xie, Hongtu,&Zhuang, Jie.(2011).Temporal responses of soil microorganisms to substrate addition as indicated by amino sugar differentiation.SOIL BIOLOGY & BIOCHEMISTRY,43(6),1155-1161. |
| MLA | He, Hongbo,et al."Temporal responses of soil microorganisms to substrate addition as indicated by amino sugar differentiation".SOIL BIOLOGY & BIOCHEMISTRY 43.6(2011):1155-1161. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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