Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS
文献类型：期刊论文
| 作者 | Li Guo-chen2; Wang Yan-hong2; Wu Ren-an1; Wang Shi-cheng2 |
| 刊名 | CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
 |
| 出版日期 | 2011-05-01 |
| 卷号 | 27期号:3页码:371-374 |
| 关键词 | Phospholipid Phosphatidylcholine Phosphatidylethanolamine Sphingomyelin Hplc Maldi Crucian Carp |
| ISSN号 | 1005-9040 |
| 英文摘要 | The interest in the analysis of phospholipids(PLs), especially phosphatidylcholine(PC), has been increasing due to the importance of them in biochemistry as well as in industry. A method was reported based on the offline combination of MALDI-TOF MS and normal-phase HPLC for analyzing PLs extracted from crucian carp. Total PLs of crucian carp were extracted and then separated by HPLC before the collected subfractions were analyzed by MALDI-TOF MS. The mass spectra obtained show peaks of H(+), Na(+) and K(+) adducts of PC molecules. It is shown that the prior separation of PLs by HPLC is highly necessary to remove the signal suppressing and to avoid the possible overlapping. With this method, 9 possible PC molecules in crucian carp and the corresponding fatty acid compositions were given from the well-resolved mass spectra. |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000291505100007 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://210.72.129.5/handle/321005/121753]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Wang Yan-hong |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Inspect & Testing Ctr Agroprod Safety & Environm, Inst Appl Ecol, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Guo-chen,Wang Yan-hong,Wu Ren-an,et al. Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2011,27(3):371-374. |
| APA | Li Guo-chen,Wang Yan-hong,Wu Ren-an,&Wang Shi-cheng.(2011).Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,27(3),371-374. |
| MLA | Li Guo-chen,et al."Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 27.3(2011):371-374. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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