The functional roles of the residue tyrosine at position 26 in staphylococcal enterotoxin C2
文献类型：期刊论文
| 作者 | Wang, Hongbo2,3; Zhou, Junyi1; Xu, Mingkai2; Zhang, Huiwen2; Zhang, Chenggang2 |
| 刊名 | AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH
 |
| 出版日期 | 2011-08-18 |
| 卷号 | 5期号:16页码:2329-2337 |
| 关键词 | Staphylococcal Enterotoxin C2 Site-directed Mutagenesis Superantigen Serological Property |
| ISSN号 | 1996-0808 |
| 英文摘要 | Bacterial superantigen Staphylococcus aureus enterotoxin C2 (SEC2) is a very potent activator of T cells. Previous crystal studies on SEC2 showed that residue Tyr26 which is located near the T cell receptor (TCR) binding sites could potentially participate in T cell activating. In SEC1, a superantigen highly homologous to SEC2, the residue Val26 played a critical role in stimulating certain V beta expansion. Moreover, residues 20, 22 and 26 in SEC1 determined its serologic cross-reaction. In order to investigate the potential roles of Tyr26 in SEC2, two SEC2 mutants SEC2(Y26V) and SEC2(Y26A) were constructed by site-directed mutagenesis. The activities and toxicities of the two mutants were determined both in vivo and in vitro. Results showed that both SEC2(Y26V) and SEC2(Y26A) remained potent immune stimulating activities compared with native SEC2. SEC2(Y26V) had a decreased pyrogenic effect on rabbit model. Both of the two mutants had significantly decreased binding affinities to anti-SEC2 IgG. Our result indicated that Tyr26 is a critical site for the serological characters of SEC2 rather than for its superantigen activity. This may also provide an insight in constructing a novel mutant to substitute native SEC2 in cancer immunotherapy avoiding being neutralized by anti-SEC2 IgG in vivo. |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000298918200022 |
| 出版者 | ACADEMIC JOURNALS |
| 源URL | [http://210.72.129.5/handle/321005/121787]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Xu, Mingkai |
| 作者单位 | 1.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hongbo,Zhou, Junyi,Xu, Mingkai,et al. The functional roles of the residue tyrosine at position 26 in staphylococcal enterotoxin C2[J]. AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,2011,5(16):2329-2337. |
| APA | Wang, Hongbo,Zhou, Junyi,Xu, Mingkai,Zhang, Huiwen,&Zhang, Chenggang.(2011).The functional roles of the residue tyrosine at position 26 in staphylococcal enterotoxin C2.AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH,5(16),2329-2337. |
| MLA | Wang, Hongbo,et al."The functional roles of the residue tyrosine at position 26 in staphylococcal enterotoxin C2".AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH 5.16(2011):2329-2337. |
入库方式： OAI收割
来源：沈阳应用生态研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。