Production of antioxidant and antitumor metabolites by submerged cultures of Inonotus obliquus cocultured with Phellinus punctatus
文献类型：期刊论文
| 作者 | Zheng, Weifa2; Zhao, Yanxia2; Zheng, Xin2; Liu, Yubing1,2; Pan, Shenyuan1; Dai, Yucheng3; Liu, Fuming4 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 89期号:1页码:157-167 |
| 关键词 | Inonotus Obliquus Phellinus Punctatus Incompatible Interactions Coculture Nmr-based Metabolomic Analysis Bioactive Metabolites |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-010-2846-2 |
| 英文摘要 | While Inonotus obliquus produces a diverse range of bioactive metabolites in its natural habitats, it accumulates less in its submerged cultures. We show here that coculture of I. obliquus with Phellinus punctatus resulted in less production of mycelial biomass but an increased accumulation of phenolic compounds, melanins, and lanostane-type triterpenoids. Metabolites increased in production by coculture include phelligridin C, phelligridin H, methyl inoscavin A, inoscavin C, inoscavin B, davallialactone, methyl davallialactone, foscoparianol D, 21,24-cyclopentalanosta-3 beta, 21,25-triol-8-en, lanosta-7,9(11),23-triene-3 beta,22,25-triol, and inotodisaccharide and melanins. Metabolites from coculture also showed an increased potential for scavenging free radicals and inhibiting the proliferation of HeLa 229 cells. Davallialactone, methyl davallialactone, and minor phenolic components are the major contributors for scavenging DPPH and hydroxyl radical in monoculture, and phelligridin C, phelligridin H, methyl inoscavin A, inoscavin C, methyl davallialactone, foscoparianol D, and inotodisaccharide are those for scavenging the tested radicals in coculture. Lanostane-type triterpenoids indicated limited roles in scavenging free radicals. Nearly all the detected metabolites correlate positively with inhibiting proliferation of HeLa 229 cells. Thus, coculture of I. obliquus with other fungi seems to be a cost-effective strategy for upregulating biosynthesis of bioactive metabolites. |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000285884200016 |
| 出版者 | SPRINGER |
| 源URL | [http://210.72.129.5/handle/321005/121863]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Zheng, Weifa |
| 作者单位 | 1.Xuzhou Normal Univ, Sch Life Sci, Xuzhou 221116, Peoples R China 2.Xuzhou Normal Univ, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Peoples R China 3.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China 4.Xuzhou Med Coll, Affiliated Hosp, Lab Ctr, Xuzhou 221009, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Weifa,Zhao, Yanxia,Zheng, Xin,et al. Production of antioxidant and antitumor metabolites by submerged cultures of Inonotus obliquus cocultured with Phellinus punctatus[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2011,89(1):157-167. |
| APA | Zheng, Weifa.,Zhao, Yanxia.,Zheng, Xin.,Liu, Yubing.,Pan, Shenyuan.,...&Liu, Fuming.(2011).Production of antioxidant and antitumor metabolites by submerged cultures of Inonotus obliquus cocultured with Phellinus punctatus.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,89(1),157-167. |
| MLA | Zheng, Weifa,et al."Production of antioxidant and antitumor metabolites by submerged cultures of Inonotus obliquus cocultured with Phellinus punctatus".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 89.1(2011):157-167. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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