QuikChange shuffling: a convenient and robust method for site-directed mutagenesis and random recombination of homologous genes
文献类型：期刊论文
| 作者 | An, Yingfeng1,3; Chen, Liming4; Sun, Shouhui2; Lv, Anguo3; Wu, Wenfang3 |
| 刊名 | NEW BIOTECHNOLOGY
 |
| 出版日期 | 2011-07-01 |
| 卷号 | 28期号:4页码:320-325 |
| ISSN号 | 1871-6784 |
| DOI | 10.1016/j.nbt.2011.03.001 |
| 英文摘要 | Here we describe a robust method, termed QuikChange shuffling, for efficient site-directed mutagenesis and random recombination of homologous genes. The homologous genes are fragmented, and the random fragments are reassembled in a self-priming polymerase reaction to obtain chimeric genes. The product is then mixed with linearized vector and two pairs of complementary mutagenic primers, followed by assembly of the chimeric genes and linearized vector through QuikChange-like amplification to introduce recombinant plasmids with a site-directed mutation. The method, which can yield 100% chimeric genes after library construction, is more convenient and efficient than current DNA shuffling methods. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000292717500004 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://210.72.129.5/handle/321005/121873]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Wu, Wenfang |
| 作者单位 | 1.Shenyang Agr Univ, Coll Biotechnol, Shenyang, Peoples R China 2.Shenyang Agr Univ, Coll Forestry, Shenyang, Peoples R China 3.Chinese Acad Sci, Inst Appl Ecol, Shenyang, Peoples R China 4.NE Forestry Univ, Coll Forest, Harbin, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Yingfeng,Chen, Liming,Sun, Shouhui,et al. QuikChange shuffling: a convenient and robust method for site-directed mutagenesis and random recombination of homologous genes[J]. NEW BIOTECHNOLOGY,2011,28(4):320-325. |
| APA | An, Yingfeng,Chen, Liming,Sun, Shouhui,Lv, Anguo,&Wu, Wenfang.(2011).QuikChange shuffling: a convenient and robust method for site-directed mutagenesis and random recombination of homologous genes.NEW BIOTECHNOLOGY,28(4),320-325. |
| MLA | An, Yingfeng,et al."QuikChange shuffling: a convenient and robust method for site-directed mutagenesis and random recombination of homologous genes".NEW BIOTECHNOLOGY 28.4(2011):320-325. |
入库方式： OAI收割
来源：沈阳应用生态研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。