Investigation of the molecular ion structure for aldononitrile acetate derivatized muramic acid
文献类型：期刊论文
| 作者 | Liang, Chao1,4; Zhang, Xudong5; Wei, Liping2,4; He, Hongbo5; Higbee, Alan J.3,4; Balser, Teri C.1 |
| 刊名 | JOURNAL OF MICROBIOLOGICAL METHODS
 |
| 出版日期 | 2011-08-01 |
| 卷号 | 86期号:2页码:224-230 |
| 关键词 | Muramic Acid Bacteria Biomarker Mass Spectrometry |
| ISSN号 | 0167-7012 |
| DOI | 10.1016/j.mimet.2011.05.007 |
| 英文摘要 | The muramic acid assay is a powerful tool for detecting both intact bacteria and bacterial debris. Past use of aldononitrile acetate derivatization for determining muramic acid in complex samples by gas chromatography/mass spectrometry met detection needs in many instances; however, questions have been raised regarding the interpretation of the derivative structure and its electron ionization fragments. In this study, we applied different methods and proved that the aldononitrile acetate derivatized muramic acid yields a molecular weight of 398, associated with a lactam structure. We also presented evidence that the structure of aldononitrile acetate derivatized muramic acid is acetylated at four positions, 3 O-acetylations and 1N-acetylation. In practical manner, this communication provides a comprehensive reference to researchers using delta(13)C value or ion fragments of the muramic acid marker in biogeochemical studies. (C) 2011 Elsevier B.V. All rights reserved. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000293490700015 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://210.72.129.5/handle/321005/121967]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Liang, Chao |
| 作者单位 | 1.Univ Wisconsin, Dept Soil Sci, Madison, WI 53706 USA 2.Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA 3.Univ Wisconsin, Ctr Biotechnol, Madison, WI 53706 USA 4.Univ Wisconsin, DOE Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA 5.Chinese Acad Sci, Inst Appl Ecol, Liaoning 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Chao,Zhang, Xudong,Wei, Liping,et al. Investigation of the molecular ion structure for aldononitrile acetate derivatized muramic acid[J]. JOURNAL OF MICROBIOLOGICAL METHODS,2011,86(2):224-230. |
| APA | Liang, Chao,Zhang, Xudong,Wei, Liping,He, Hongbo,Higbee, Alan J.,&Balser, Teri C..(2011).Investigation of the molecular ion structure for aldononitrile acetate derivatized muramic acid.JOURNAL OF MICROBIOLOGICAL METHODS,86(2),224-230. |
| MLA | Liang, Chao,et al."Investigation of the molecular ion structure for aldononitrile acetate derivatized muramic acid".JOURNAL OF MICROBIOLOGICAL METHODS 86.2(2011):224-230. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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