An engineered superantigen SEC2 exhibits promising antitumor activity and low toxicity
文献类型：期刊论文
| 作者 | Xu, Mingkai2; Wang, Xiaogang2; Cai, Yongming3; Zhang, Huiwen2; Yang, Hongli1; Liu, Changxiao3; Zhang, Chenggang2 |
| 刊名 | CANCER IMMUNOLOGY IMMUNOTHERAPY
 |
| 出版日期 | 2011-05-01 |
| 卷号 | 60期号:5页码:705-713 |
| 关键词 | Staphylococcal Enterotoxin C2 Site-directed Mutagenesis Superantigen Immunotherapy |
| ISSN号 | 0340-7004 |
| DOI | 10.1007/s00262-011-0986-6 |
| 英文摘要 | Recent studies suggested that the histidine residues at 118 and 122 play an important role for the toxicity of staphylococcal enterotoxin C subtype 2 (SEC2), and the substitutions of both histidines with alanine can severely impair the fever activity of SEC2. We hypothesized that promising SEC2 antitumor agent with low toxicity and enhanced superantigen activity can be constructed by introducing related mutations at protein functional sites of SEC2. We showed that the SEC2 mutants H122A and H118A/H122A exhibited improved superantigen activity after introducing the point mutations at Thr20 and Gly22. A resultant mutant, named as SAM-3, has considerable abilities to inhibit the growth of H22 and Hepa1-6 tumor cells in vitro and colon 26 solid tumor in vivo. Furthermore, SAM-3 also exhibits significantly reduced toxicity compared with native SEC2. The study provides a novel strategy for designing promising superantigen immunotherapeutic agent. The constructed SEC2 mutant SAM-3 can be used as a powerful candidate for cancer immunotherapy and could compensate the deficiency caused by toxicity of native SEC2 in clinic. |
| WOS研究方向 | Oncology ; Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000289728500010 |
| 出版者 | SPRINGER |
| 源URL | [http://210.72.129.5/handle/321005/121987]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Wang, Xiaogang |
| 作者单位 | 1.Shenyang Xiehe Biopharmaceut Co Ltd, Shenyang 110179, Peoples R China 2.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China 3.Tianjin Inst Pharmaceut Res, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Mingkai,Wang, Xiaogang,Cai, Yongming,et al. An engineered superantigen SEC2 exhibits promising antitumor activity and low toxicity[J]. CANCER IMMUNOLOGY IMMUNOTHERAPY,2011,60(5):705-713. |
| APA | Xu, Mingkai.,Wang, Xiaogang.,Cai, Yongming.,Zhang, Huiwen.,Yang, Hongli.,...&Zhang, Chenggang.(2011).An engineered superantigen SEC2 exhibits promising antitumor activity and low toxicity.CANCER IMMUNOLOGY IMMUNOTHERAPY,60(5),705-713. |
| MLA | Xu, Mingkai,et al."An engineered superantigen SEC2 exhibits promising antitumor activity and low toxicity".CANCER IMMUNOLOGY IMMUNOTHERAPY 60.5(2011):705-713. |
入库方式： OAI收割
来源：沈阳应用生态研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。