中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 沈阳应用生态研究所
  3. 中国科学院沈阳应用生态研究所
DNA damage and genetic methylation changes caused by Cd in Arabidopsis thaliana seedlings

文献类型：期刊论文

作者Jia, Chunyun2; Li, Zhaoling1,2,3; Liu, Zhihong2,4; Chen, Ruijuan2,3; Li, Xiaojun2; Tai, Peidong2; Gong, Zongqiang2; Liu, Wan2
刊名ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
出版日期2015-09-01
卷号34期号:9页码:2095-2103
关键词Amplified Fragment Length Polymorphism Methylation-sensitive Amplification Polymorphism Dna Damage Genetic Methylation Cadmium
ISSN号0730-7268
DOI10.1002/etc.3033
英文摘要Amplified fragment length polymorphism (AFLP) and methylation-sensitive amplification polymorphism (MASP) techniques are sensitive to deoxyribonucleic acid (DNA) damage and genetic methylation, respectively. Using these 2 techniques, Arabidopsis thaliana cultured with 0mg/L (control), 0.5mg/L, 1.5mg/L, and 5.0mg/L Cd2+ for 16d was used to analyze the DNA damage and methylation changes as a result of cadmium (Cd). The DNA was amplified by 14 AFLP primer pairs and 13 MSAP primer combinations. In the AFLP experiment, 62 polymorphic sites were found in the patterns of 11 primer combinations and a total of 1116 fragments were obtained in these patterns. There were no polymorphic bands in the remaining 3 pairs. The proportions of polymorphic sites in the 0.5-mg/L Cd2+ and 5.0-mg/L Cd2+ treatments were significantly different. Seven polymorphic fragments were then separated and successfully sequenced, yielding 6 nucleobase substitutions and 1 nucleobase deletion. Similarly, in the MSAP experiment, the MSAP% and number of demethylated-type bands were unchanged after Cd treatment, but the number of methylated-type bands was increased significantly in the 5.0-mg/L Cd2+ treatment group, a finding that may be associated with the AFLP results. The polymorphic bands were also sequenced and the functions of their homologous genes were determined. The DNA damage and methylation changes may be the primary cause of certain pathology changes as a result of Cd uptake in plants. Environ Toxicol Chem 2015;34:2095-2103. (c) 2015 SETAC
WOS研究方向Environmental Sciences & Ecology ; Toxicology
语种英语
WOS记录号WOS:000360500600024
出版者WILEY-BLACKWELL
源URL[http://210.72.129.5/handle/321005/122167]  
专题中国科学院沈阳应用生态研究所
通讯作者Liu, Wan
作者单位1.Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki, Japan
2.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Liaoning, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Deakin Univ, Sch Life & Environm, Warrnambool, Vic, Australia
推荐引用方式
GB/T 7714		 Jia, Chunyun,Li, Zhaoling,Liu, Zhihong,et al. DNA damage and genetic methylation changes caused by Cd in Arabidopsis thaliana seedlings[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2015,34(9):2095-2103.
APA Jia, Chunyun.,Li, Zhaoling.,Liu, Zhihong.,Chen, Ruijuan.,Li, Xiaojun.,...&Liu, Wan.(2015).DNA damage and genetic methylation changes caused by Cd in Arabidopsis thaliana seedlings.ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,34(9),2095-2103.
MLA Jia, Chunyun,et al."DNA damage and genetic methylation changes caused by Cd in Arabidopsis thaliana seedlings".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 34.9(2015):2095-2103.

入库方式： OAI收割

来源：沈阳应用生态研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。