Enhanced rhamnolipid production of Pseudomonas aeruginosa SG by increasing copy number of rhlAB genes with modified promoter
文献类型：期刊论文
| 作者 | Zhang, Jie2; Zhang, Ying1; Zhao, Feng1,2; Cui, Qingfeng3; Han, Siqin1; Dong, Hanping3; Ma, Fang2 |
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2015 |
| 卷号 | 5期号:86页码:70546-70552 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c5ra13415c |
| 英文摘要 | Rhamnolipid is a potent natural biosurfactant for industrial and environmental applications such as enhanced oil recovery, bioremediation, and biodegradation. The complex regulation network of key genes involved in the biosynthesis of rhamnolipid in Pseudomonas aeruginosa represents a challenge to the industrial production of rhamnolipid. In this study, rhlAB genes with native promoter were cloned from P. aeruginosa SG and inserted into plasmid pBBR1MCS-5 to construct recombinant plasmid pBBRPrhlAB. And rhlAB genes fused with the strong promoter of oprL gene from P. aeruginosa SG were used to construct recombinant plasmid pBBRPoprAB. Two recombinant plasmids were transformed to strain SG to construct the engineered strains PrhlAB and PoprAB. Both strains PrhlAB and PoprAB have higher yield of rhamnolipid than wild strain SG under both aerobic and anaerobic conditions. Increasing the copy number of rhlAB genes with indigenous strong promoter of oprL gene, engineered strain PoprAB has the highest yield of rhamnolipid (1.83-fold of strain SG and 1.19-fold of strain PrhlAB) under aerobic conditions. It is more efficient, economic and commercially feasible to use the engineered strain PoprAB for rhamnolipid production. Using the promoter of oprL gene to enhance production of other desirable product in P. aeruginosa may also be feasible. |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000360404400084 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://210.72.129.5/handle/321005/122169]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Ma, Fang |
| 作者单位 | 1.Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Peoples R China 2.Harbin Inst Technol, Sch Municipal & Environm Engn, State Key Lab Urban Water Resource & Environm, Harbin 150090, Peoples R China 3.Res Inst Petr Explorat & Dev Langfang, Inst Porous Flow & Fluid Mech, Langfang 065007, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jie,Zhang, Ying,Zhao, Feng,et al. Enhanced rhamnolipid production of Pseudomonas aeruginosa SG by increasing copy number of rhlAB genes with modified promoter[J]. RSC ADVANCES,2015,5(86):70546-70552. |
| APA | Zhang, Jie.,Zhang, Ying.,Zhao, Feng.,Cui, Qingfeng.,Han, Siqin.,...&Ma, Fang.(2015).Enhanced rhamnolipid production of Pseudomonas aeruginosa SG by increasing copy number of rhlAB genes with modified promoter.RSC ADVANCES,5(86),70546-70552. |
| MLA | Zhang, Jie,et al."Enhanced rhamnolipid production of Pseudomonas aeruginosa SG by increasing copy number of rhlAB genes with modified promoter".RSC ADVANCES 5.86(2015):70546-70552. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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