Production and Characterization of Surfactin-Type Lipopeptides as Bioemulsifiers Produced by a Pinctada martensii-Derived Bacillus mojavensis B0621A
文献类型：期刊论文
| 作者 | Ma, Zongwang1,2; Hu, Jiangchun1 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 177期号:7页码:1520-1529 |
| ISSN号 | 0273-2289 |
| 关键词 | Bacillus Mojavensis Bacillus Surfactins Bioemulsifiers Chemical Identification Emulsification Activity |
| DOI | 10.1007/s12010-015-1832-7 |
| 英文摘要 | Bacillus mojavensis B0621A was isolated from the mantle of a pearl oyster Pinctada martensii collected from South China Sea. Semi-purified surfactins (225 mg L-1) were obtained by acid precipitation and vacuum flash chromatography. The component of the semi-purified surfactins was preliminarily analyzed by liquid chromatography mass spectrometer system, and the results showed that all these surfactins could be a group of homologues. Eight surfactin homologues were isolated and afforded by reversed phase high-performance liquid chromatography. Furthermore, their structure was characterized by mass spectrometry analysis combined with nuclear magnetic resonance spectroscopy techniques. These surfactins shared seven amino acids as peptide backbone and a saturated beta-hydroxy fatty acid chain residue (from C-13 to C-15), differed each other from peptide sequence in the position of Leu(7) or Val(7). All these surfactins had significant activity and stability of emulsification under various pH (from 7.0 to 12.0), temperature range (from 20 to 115 A degrees C) and sodium chloride concentration (from 2.5 to 20.0 %, w/v). Taken all together, these results indicated that B. mojavensis B0621A have potential to be an alternative source as a biological-derived emulsifying agent. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | HUMANA PRESS INC |
| WOS记录号 | WOS:000365165500011 |
| 源URL | [http://210.72.129.5/handle/321005/122285]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Hu, Jiangchun |
| 作者单位 | 1.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Zongwang,Hu, Jiangchun. Production and Characterization of Surfactin-Type Lipopeptides as Bioemulsifiers Produced by a Pinctada martensii-Derived Bacillus mojavensis B0621A[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2015,177(7):1520-1529. |
| APA | Ma, Zongwang,&Hu, Jiangchun.(2015).Production and Characterization of Surfactin-Type Lipopeptides as Bioemulsifiers Produced by a Pinctada martensii-Derived Bacillus mojavensis B0621A.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,177(7),1520-1529. |
| MLA | Ma, Zongwang,et al."Production and Characterization of Surfactin-Type Lipopeptides as Bioemulsifiers Produced by a Pinctada martensii-Derived Bacillus mojavensis B0621A".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 177.7(2015):1520-1529. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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