Microbial availability of mercury: effective detection and organic ligand effect using a whole-cell bioluminescent bioreporter
文献类型：期刊论文
| 作者 | Xu, Xianghua1,2; Oliff, Kathryn2; Xu, Tingting2; Ripp, Steven2; Sayler, Gary2; Zhuang, Jie3 |
| 刊名 | ECOTOXICOLOGY
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 24期号:10页码:2200-2206 |
| 关键词 | Mercury Bioavailability Humic Acids Edta Bioreporter Escherichia Coli Arl1 |
| ISSN号 | 0963-9292 |
| DOI | 10.1007/s10646-015-1553-2 |
| 英文摘要 | A luxCDABE-based genetically engineered bacterial bioreporter (Escherichia coli ARL1) was used to detect bioavailable ionic mercury (Hg(II)) and investigate the effects of humic acids and ethylenediaminetetraacetic acid (EDTA) on the bioavailability of mercury in E. c oli. Results showed that the E. c oli ARL1 bioreporter was sensitive to mercury, with a detection limit of Hg(II) of 0.5 A mu g/L and a linear dose/response relationship up to 2000 A mu g Hg(II)/L. Humic acids and EDTA decreased the Hg(II)-induced bioluminescent response of strain ARL1, suggesting that the two organic ligands reduced the bioavailability of Hg(II) via complexation with Hg(II). Compared with traditional chemical methods, the use of E. c oli ARL1 is a cost-effective, rapid, and reliable approach for measuring aqueous mercury at very low concentrations and thus has potential for applications in field in situ monitoring. |
| WOS研究方向 | Environmental Sciences & Ecology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000365758200018 |
| 出版者 | SPRINGER |
| 源URL | [http://210.72.129.5/handle/321005/122313]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Zhuang, Jie |
| 作者单位 | 1.Nanjing Univ Informat Sci & Technol, Jiangsu Key Lab Agr Meteorol, Nanjing 210044, Jiangsu, Peoples R China 2.Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37996 USA 3.Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Xianghua,Oliff, Kathryn,Xu, Tingting,et al. Microbial availability of mercury: effective detection and organic ligand effect using a whole-cell bioluminescent bioreporter[J]. ECOTOXICOLOGY,2015,24(10):2200-2206. |
| APA | Xu, Xianghua,Oliff, Kathryn,Xu, Tingting,Ripp, Steven,Sayler, Gary,&Zhuang, Jie.(2015).Microbial availability of mercury: effective detection and organic ligand effect using a whole-cell bioluminescent bioreporter.ECOTOXICOLOGY,24(10),2200-2206. |
| MLA | Xu, Xianghua,et al."Microbial availability of mercury: effective detection and organic ligand effect using a whole-cell bioluminescent bioreporter".ECOTOXICOLOGY 24.10(2015):2200-2206. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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