Microtubule-Associated Protein SBgLR Facilitates Storage Protein Deposition and Its Expression Leads to Lysine Content Increase in Transgenic Maize Endosperm
文献类型：期刊论文
| 作者 | Yue, Jing2; Liu, Chen1,2; Li, Shixue2; Xiao, Wenhan2; Zhao, Qian2; Zhu, Dengyun2; Yu, Jingjuan2 |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 16期号:12页码:29772-29786 |
| ISSN号 | 1422-0067 |
| 关键词 | Sbglr Microtubule-associated Protein Protein Body Formation Maize Nutrient Improvement |
| DOI | 10.3390/ijms161226199 |
| 英文摘要 | Maize (Zea mays) seed is deficient in protein and lysine content. Many studies have been made to improve the nutritional quality of maize seeds. Previously, we reported the role of a natural lysine-rich protein gene SBgLR in increasing protein and lysine content. However, how the SBgLR improves lysine and protein content remains unclear. Here, the reasons and possible mechanism for SBgLR in protein and lysine improvement have been analyzed and discussed. Through seed-specific expression of SBgLR, we obtained transgenic maize with the simultaneously increased lysine and protein contents. High-protein and high-lysine characters were stably inherited across generations. The expression of SBgLR in maize kernels increased the accumulation of both zeins and non-zein proteins. Transmission electron microscopy showed that the number of protein bodies (PBs) was increased obviously in SBgLR transgenic immature endosperms with the morphology and structure of PBs unchanged. The proteinaceous matrix was more abundant in transgenic mature endosperms under scanning electron microscopy. The stabilities of zein and lysine-rich non-zein genes were also increased in transgenic endosperms. Finally, the potential application of SBgLR in maize nutrient improvement was evaluated. This study shows that a cytoskeleton-associated protein has potential applicable value in crop nutrient improving, and provided a feasible strategy for improvement of maize grain quality. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | MDPI AG |
| WOS记录号 | WOS:000367535600116 |
| 源URL | [http://210.72.129.5/handle/321005/122379]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Yu, Jingjuan |
| 作者单位 | 1.Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Peoples R China 2.China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yue, Jing,Liu, Chen,Li, Shixue,et al. Microtubule-Associated Protein SBgLR Facilitates Storage Protein Deposition and Its Expression Leads to Lysine Content Increase in Transgenic Maize Endosperm[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2015,16(12):29772-29786. |
| APA | Yue, Jing.,Liu, Chen.,Li, Shixue.,Xiao, Wenhan.,Zhao, Qian.,...&Yu, Jingjuan.(2015).Microtubule-Associated Protein SBgLR Facilitates Storage Protein Deposition and Its Expression Leads to Lysine Content Increase in Transgenic Maize Endosperm.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,16(12),29772-29786. |
| MLA | Yue, Jing,et al."Microtubule-Associated Protein SBgLR Facilitates Storage Protein Deposition and Its Expression Leads to Lysine Content Increase in Transgenic Maize Endosperm".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 16.12(2015):29772-29786. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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