Weathering of pyrogenic organic matter induces fungal oxidative enzyme response in single culture inoculation experiments
文献类型：期刊论文
| 作者 | Wang, Ruzhen1,2,3; Gibson, Christy1,2; Berry, Timothy D.1,2; Spencer, Julie A.4; Johnston, Cliff T.5; Jiang, Yong3; Bird, Jeffrey A.6,7; Filley, Timothy R.1,2,5 |
| 刊名 | ORGANIC GEOCHEMISTRY
 |
| 出版日期 | 2016-02-01 |
| 卷号 | 92页码:32-41 |
| 关键词 | C-13 Pyrogenic Organic Matter Fungal Decomposition Enzyme Phenol Oxidase Peroxidase Priming |
| ISSN号 | 0146-6380 |
| DOI | 10.1016/j.orggeochem.2015.12.003 |
| 英文摘要 | The addition of pyrogenic organic matter (PyOM), the aromatic carbon-rich product of the incomplete combustion of plant biomass or fossil fuels, to soil can influence the rate of microbial metabolism of native soil carbon. The interaction of soil heterotrophs with PyOM may be governed by the surficial chemical and physical properties of PyOM that evolve with environmental exposure. We present results of a 36-day laboratory incubation investigating the interaction of a common white-rot fungus, Trametes versicolor, with three forms of C-13-enriched (2.08 atom% C-13) PyOM derived from Pinus ponderosa (450 degrees C): one freshly produced, and two artificially weathered (254 nm, UV light-water treatment and water-leaching alone). Analysis (FTIR, XPS) of the UV-weathered PyOM showed increased aliphatic C-H content and oxidation of aromatic carbon relative to both the original and water-leached PyOM. The addition of both weathered forms of PyOM stimulated (positively primed) fungal respiration of the growth media, while the unaltered PyOM mildly inhibited (negatively primed) respiration. Artificial weathering resulted in higher oxidative (laccase and peroxidase) enzyme activity than unaltered PyOM, possibly the result of a diminished capacity to bind reactive substrates and extracellular enzymes after weathering. However, and contrary to expectations, simple water-leached weathering resulted in a relatively higher enzyme activity and respiration than that of UV-weathering. The C-13 content of respired CO2 indicated negligible fungal oxidation of PyOM for all treatments, demonstrating the overall low microbial reactivity of this high temperature PyOM. The increased enzymatic and positive priming response of T. versicolor to weathered PyOM highlights the importance of weathering-induced chemistry in controlling PyOM-microbe-soil carbon interactions. (C) 2015 Elsevier Ltd. All rights reserved. |
| WOS研究方向 | Geochemistry & Geophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000368560600004 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://210.72.129.5/handle/321005/122407]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Filley, Timothy R. |
| 作者单位 | 1.Purdue Univ, Dept Earth Atmospher & Planetary Sci, W Lafayette, IN 47907 USA 2.Purdue Univ, Purdue Climate Change Res Ctr, W Lafayette, IN 47907 USA 3.Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110016, Peoples R China 4.Johns Hopkins Univ, Dept Chem, Baltimore, MD 21218 USA 5.Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA 6.CUNY Queens Coll, Sch Earth & Environm Sci, Flushing, NY 11367 USA 7.CUNY, Grad Ctr, Earth & Environm Sci PhD Program, New York, NY 10016 USA |
| 推荐引用方式 GB/T 7714 | Wang, Ruzhen,Gibson, Christy,Berry, Timothy D.,et al. Weathering of pyrogenic organic matter induces fungal oxidative enzyme response in single culture inoculation experiments[J]. ORGANIC GEOCHEMISTRY,2016,92:32-41. |
| APA | Wang, Ruzhen.,Gibson, Christy.,Berry, Timothy D..,Spencer, Julie A..,Johnston, Cliff T..,...&Filley, Timothy R..(2016).Weathering of pyrogenic organic matter induces fungal oxidative enzyme response in single culture inoculation experiments.ORGANIC GEOCHEMISTRY,92,32-41. |
| MLA | Wang, Ruzhen,et al."Weathering of pyrogenic organic matter induces fungal oxidative enzyme response in single culture inoculation experiments".ORGANIC GEOCHEMISTRY 92(2016):32-41. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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