TNF-alpha produced by SEC2 mutant (SAM-3)-activated human T cells induces apoptosis of HepG2 cells
文献类型：期刊论文
| 作者 | Zhang, Huiwen1; Su, Zhencheng1; Zhang, Chenggang1; Zhang, Guojun1,2; Xu, Mingkai1; Song, Yubo1,2 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2016-03-01 |
| 卷号 | 100期号:6页码:2677-2684 |
| 关键词 | Superantigen Staphylococcal Enterotoxin C2 Antitumor Apoptosis Tnf-alpha |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-015-7104-1 |
| 英文摘要 | Staphylococcal enterotoxins C2 (SEC2) is a classical model of superantigens (SAg), which has the powerful ability to activate T cells as well as induce massive cytokine production. This property makes SEC2 and its mutants well concerned as a potential new immune-regulatory agent for cancer therapy. We previously constructed a SEC2 mutant named SAM-3, which had prominently antitumor activity in BALB/c mice model. But, the underlying molecular mechanism for stimulation of human peripheral blood mononuclear cells (PBMCs) and antitumor effect on human tumor cells induced by SAM-3 is not clear. Here, we showed that SAM-3 could activate human TCR V beta 12, 13A, 14, 15, 17, and 20 CD8(+) subgroup T cells, which secreted the cytokines IL-2, IFN-gamma, and TNF-alpha, and exhibit stimulation activity in a dose-dependent manner. TNF-alpha secreted from activated T cells could induce apoptosis and G1-phase arrest and lead to the antitumor effect in HepG2 cells. Meanwhile, SAM-3 upregulated the expression of tumor necrosis factor receptor 1 (TNFR1) mRNA and activity of caspase-3 and caspase-8. We also found that the antitumor activity and activity of caspase-3 and caspase-8 were decreased when the neutralizing TNF-alpha monoclonal antibody presented. These data suggest that TNF-alpha secreted by SAM-3-activated T cells is an important factor in inducing apoptosis in HepG2 cells. |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000371244300015 |
| 出版者 | SPRINGER |
| 源URL | [http://210.72.129.5/handle/321005/122499]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Zhang, Huiwen |
| 作者单位 | 1.Chinese Acad Sci, Inst Appl Ecol, 72 Wenhua Rd, Shenyang 110016, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Huiwen,Su, Zhencheng,Zhang, Chenggang,et al. TNF-alpha produced by SEC2 mutant (SAM-3)-activated human T cells induces apoptosis of HepG2 cells[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2016,100(6):2677-2684. |
| APA | Zhang, Huiwen,Su, Zhencheng,Zhang, Chenggang,Zhang, Guojun,Xu, Mingkai,&Song, Yubo.(2016).TNF-alpha produced by SEC2 mutant (SAM-3)-activated human T cells induces apoptosis of HepG2 cells.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,100(6),2677-2684. |
| MLA | Zhang, Huiwen,et al."TNF-alpha produced by SEC2 mutant (SAM-3)-activated human T cells induces apoptosis of HepG2 cells".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 100.6(2016):2677-2684. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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