Cadmium-induced genomic instability in Arabidopsis: Molecular toxicological biomarkers for early diagnosis of cadmium stress
文献类型：期刊论文
| 作者 | Sun, Lizong1; Hui, Xiujuan3; Wang, Hetong1,7; He, Lei1,2; Song, Jie1,2; Cui, Weina1,3; Liu, Wan1; Yang, Yuesuo5; Zhang, Yanzhao4; Jia, Chunyun1 |
| 刊名 | CHEMOSPHERE
 |
| 出版日期 | 2016-05-01 |
| 卷号 | 150页码:258-265 |
| ISSN号 | 0045-6535 |
| 关键词 | Microsatellite Instability Random-amplified Polymorphic Dna Methylation-sensitive Arbitrarily Primed Pcr Cadmium Arabidopsis Thaliana |
| DOI | 10.1016/j.chemosphere.2016.02.042 |
| 英文摘要 | Microsatellite instability (MSI) analysis, random-amplified polymorphic DNA (RAPD), and methylation-sensitive arbitrarily primed PCR (MSAP-PCR) are methods to evaluate the toxicity of environmental pollutants in stress-treated plants and human cancer cells. Here, we evaluate these techniques to screen for genetic and epigenetic alterations of Arabidopsis plantlets exposed to 0-5.0 mg L-1 cadmium (Cd) for 15 d. There was a substantial increase in RAPD polymorphism of 24.5, and in genomic methylation polymorphism of 30.5-34.5 at CpG and of 14.5-20 at CHG sites under Cd stress of 5.0 mg L-1 by RAPD and of 0.25-5.0 mg L-1 by MSAP-PCR, respectively. However, only a tiny increase of 1.5 loci by RAPD occurred under Cd stress of 4.0 mg L-1, and an additional high dose (8.0 mg L-1) resulted in one repeat by MSI analysis. MSAP-PCR detected the most significant epigenetic modifications in plantlets exposed to Cd stress, and the patterns of hypermethylation and polymorphisms were consistent with inverted U-shaped dose responses. The presence of genomic methylation polymorphism in Cd-treated seedlings, prior to the onset of RAPD polymorphism, MSI and obvious growth effects, suggests that these altered DNA methylation loci are the most sensitive biomarkers for early diagnosis and risk assessment of genotoxic effects of Cd pollution in ecotoxicology. (C) 2016 Elsevier Ltd. All rights reserved. |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000372765100033 |
| 源URL | [http://210.72.129.5/handle/321005/122538]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Liu, Wan |
| 作者单位 | 1.Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Peoples R China 2.Liao Univ, Environm Sci Coll, Shenyang 110036, Peoples R China 3.Shanghai Inst Technol, Shanghai 201418, Peoples R China 4.Luoyang Normal Univ, Dept Life Sci, Luoyang 471022, Peoples R China 5.Shenyang Univ, Key Lab Ecorestorat, Shenyang 11044, Peoples R China 6.Cardiff Univ, Sch Biosci, Cardiff CF10 33TL, S Glam, Wales 7.He Univ, Dept Basic Med, Shenyang 110163, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Lizong,Hui, Xiujuan,Wang, Hetong,et al. Cadmium-induced genomic instability in Arabidopsis: Molecular toxicological biomarkers for early diagnosis of cadmium stress[J]. CHEMOSPHERE,2016,150:258-265. |
| APA | Sun, Lizong.,Hui, Xiujuan.,Wang, Hetong.,He, Lei.,Song, Jie.,...&Tai, Peidong.(2016).Cadmium-induced genomic instability in Arabidopsis: Molecular toxicological biomarkers for early diagnosis of cadmium stress.CHEMOSPHERE,150,258-265. |
| MLA | Sun, Lizong,et al."Cadmium-induced genomic instability in Arabidopsis: Molecular toxicological biomarkers for early diagnosis of cadmium stress".CHEMOSPHERE 150(2016):258-265. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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