Medium factors on anaerobic production of rhamnolipids by Pseudomonas aeruginosa SG and a simplifying medium for in situ microbial enhanced oil recovery applications
文献类型：期刊论文
| 作者 | Ma, Fang1; Zhang, Jie1; Han, Siqin2; Zhou, Jidong2; Zhang, Ying2; Zhao, Feng1,2 |
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 32期号:4 |
| 关键词 | Rhamnolipid Anaerobic Conditions Glycerol Nitrate Simplifying Medium Microbial Enhanced Oil Recovery |
| ISSN号 | 0959-3993 |
| DOI | 10.1007/s11274-016-2020-9 |
| 英文摘要 | Aerobic production of rhamnolipid by Pseudomonas aeruginosa was extensively studied. But effect of medium composition on anaerobic production of rhamnolipid by P. aeruginosa was unknown. A simplifying medium facilitating anaerobic production of rhamnolipid is urgently needed for in situ microbial enhanced oil recovery (MEOR). Medium factors affecting anaerobic production of rhamnolipid were investigated using P. aeruginosa SG (Genbank accession number KJ995745). Medium composition for anaerobic production of rhamnolipid by P. aeruginosa is different from that for aerobic production of rhamnolipid. Both hydrophobic substrate and organic nitrogen inhibited rhamnolipid production under anaerobic conditions. Glycerol and nitrate were the best carbon and nitrogen source. The commonly used N limitation under aerobic conditions was not conducive to rhamnolipid production under anaerobic conditions because the initial cell growth demanded enough nitrate for anaerobic respiration. But rhamnolipid was also fast accumulated under nitrogen starvation conditions. Sufficient phosphate was needed for anaerobic production of rhamnolipid. SO42- and Mg2+ are required for anaerobic production of rhamnolipid. Results will contribute to isolation bacteria strains which can anaerobically produce rhamnolipid and medium optimization for anaerobic production of rhamnolipid. Based on medium optimization by response surface methodology and ions composition of reservoir formation water, a simplifying medium containing 70.3 g/l glycerol, 5.25 g/l NaNO3, 5.49 g/l KH2PO4, 6.9 g/l K2HPO4 center dot 3H(2)O and 0.40 g/l MgSO4 was designed. Using the simplifying medium, 630 mg/l of rhamnolipid was produced by SG, and the anaerobic culture emulsified crude oil to EI24 = 82.5 %. The simplifying medium was promising for in situ MEOR applications. |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000373698000002 |
| 出版者 | SPRINGER |
| 源URL | [http://210.72.129.5/handle/321005/122582]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Zhang, Jie; Zhang, Ying |
| 作者单位 | 1.Harbin Inst Technol, Sch Municipal & Environm Engn, State Key Lab Urban Water Resource & Environm, Harbin 150090, Peoples R China 2.Chinese Acad Sci, Inst Appl Ecol, Key Lab Pollut Ecol & Environm Engn, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Fang,Zhang, Jie,Han, Siqin,et al. Medium factors on anaerobic production of rhamnolipids by Pseudomonas aeruginosa SG and a simplifying medium for in situ microbial enhanced oil recovery applications[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2016,32(4). |
| APA | Ma, Fang,Zhang, Jie,Han, Siqin,Zhou, Jidong,Zhang, Ying,&Zhao, Feng.(2016).Medium factors on anaerobic production of rhamnolipids by Pseudomonas aeruginosa SG and a simplifying medium for in situ microbial enhanced oil recovery applications.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,32(4). |
| MLA | Ma, Fang,et al."Medium factors on anaerobic production of rhamnolipids by Pseudomonas aeruginosa SG and a simplifying medium for in situ microbial enhanced oil recovery applications".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 32.4(2016). |
入库方式： OAI收割
来源：沈阳应用生态研究所
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