Sphingomonas from petroleum-contaminated soils in Shenfu, China and their PAHs degradation abilities
文献类型：期刊论文
| 作者 | Han, Siqin1; Zhou, Lisha1; Li, Hui1,2; Zhang, Ying1; Xu, Hui1 |
| 刊名 | BRAZILIAN JOURNAL OF MICROBIOLOGY
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 47期号:2页码:271-278 |
| 关键词 | Sphingomonas Polycyclic Aromatic Hydrocarbons (Pahs) Degradation Genus |
| ISSN号 | 1517-8382 |
| DOI | 10.1016/j.bjm.2016.01.001 |
| 英文摘要 | Members of the Sphingomonas genus are often isolated from petroleum-contaminated soils due to their unique abilities to degrade polycyclic aromatic hydrocarbons (PAHs), which are important for in situ bioremediation. In this study, a combined phenotypic and genotypic approach using streptomycin-containing medium and Sphingomonas-specific PCR was developed to isolate and identify culturable Sphingomonas strains present in petroleum contaminated soils in the Shenfu wastewater irrigation zone. Of the 15 soil samples examined, 12 soils yielded yellow streptomycin-resistant colonies. The largest number of yellow colony-forming units (CFUs) could reach 10(5) CFUs g(-1) soil. The number of yellow CFUs had a significant positive correlation (p < 0.05) with the ratio of PAHs to total petroleum hydrocarbons (TPH), indicating that Sphingomonas may play a key role in degrading the PAH fraction of the petroleum contaminants at this site. Sixty yellow colonies were selected randomly and analyzed by colony PCR using Sphingomonas-specific primers, out of which 48 isolates had PCR-positive signals. The 48 positive amplicons generated 8 distinct restriction fragment length polymorphism (RFLP) patterns, and 7 out of 8 phylotypes were identified as Sphingomonas by 16S rRNA gene sequencing of the representative strains. Within these 7 Sphingomonas strains, 6 strains were capable of using fluorene as the sole carbon source, while 2 strains were phenanthrene-degrading Sphingomonas. To the best of our knowledge, this is the first report to evaluate the relationship between PAHs contamination levels and culturable Sphingomonas in environmental samples. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000376016600001 |
| 出版者 | SOC BRASILEIRA MICROBIOLOGIA |
| 源URL | [http://210.72.129.5/handle/321005/122657]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Li, Hui |
| 作者单位 | 1.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Siqin,Zhou, Lisha,Li, Hui,et al. Sphingomonas from petroleum-contaminated soils in Shenfu, China and their PAHs degradation abilities[J]. BRAZILIAN JOURNAL OF MICROBIOLOGY,2016,47(2):271-278. |
| APA | Han, Siqin,Zhou, Lisha,Li, Hui,Zhang, Ying,&Xu, Hui.(2016).Sphingomonas from petroleum-contaminated soils in Shenfu, China and their PAHs degradation abilities.BRAZILIAN JOURNAL OF MICROBIOLOGY,47(2),271-278. |
| MLA | Han, Siqin,et al."Sphingomonas from petroleum-contaminated soils in Shenfu, China and their PAHs degradation abilities".BRAZILIAN JOURNAL OF MICROBIOLOGY 47.2(2016):271-278. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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