The Study of Organic Nitrogen Uptake by Soil Microorganisms Using Gas Chromatography-Mass Spectrometry and Double Isotope Labeling Technique
文献类型：期刊论文
| 作者 | Wang Ling-li1; Yang Li-jie1,2; Zhang Li-li1; Chen Wei1,2; Gong Ping1; Wu Zhi-jie1; Xue Yan1,2; Fang Na-na1 |
| 刊名 | SPECTROSCOPY AND SPECTRAL ANALYSIS
 |
| 出版日期 | 2016-05-01 |
| 卷号 | 36期号:5页码:1478-1482 |
| 关键词 | Compound Specific Stable Isotope Analysis Double Labeled Glycine A-keto Acid |
| ISSN号 | 1000-0593 |
| DOI | 10.3964/j.issn.1000-0593(2016)05-1478-05 |
| 英文摘要 | The direct absorption and utilization of low-molecular weight organic nitrogen (N) by soil microbial is a new subject in the research of microbial N nutrition. The study used gas chromatography-mass spectrometry to trace dual-labeled (C-13, N-15) glycine from the soil solution and microorganisms. The results showed that glycine added to the soil was quickly taken up by soil microorganisms, with the half-life of glycine being 2. 9 h. Withthe incubation of 4 h, the maximum amount of dual-labeled glycine in the microbial biomass was measured (equivalent to 10% of glycine added), indicating that added glycine was absorbed as intact molecular by soil microorganisms. The single labeled-Keto acid was detected in soil solution and in the microorganisms (decomposed production by double labeled glycine), but the content is extremely low, suggesting that added glycine mainly served as carbon (C) source for soil microbial life activities. This study demonstrated that compound specific stable dual labeled isotope analysis combined with chloroform fumigation technique was an effective method for detecting the low-molecular organic N utilized by soil microorganisms. |
| WOS研究方向 | Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000375891900037 |
| 出版者 | OFFICE SPECTROSCOPY & SPECTRAL ANALYSIS |
| 源URL | [http://210.72.129.5/handle/321005/122659]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Zhang Li-li |
| 作者单位 | 1.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang Ling-li,Yang Li-jie,Zhang Li-li,et al. The Study of Organic Nitrogen Uptake by Soil Microorganisms Using Gas Chromatography-Mass Spectrometry and Double Isotope Labeling Technique[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2016,36(5):1478-1482. |
| APA | Wang Ling-li.,Yang Li-jie.,Zhang Li-li.,Chen Wei.,Gong Ping.,...&Fang Na-na.(2016).The Study of Organic Nitrogen Uptake by Soil Microorganisms Using Gas Chromatography-Mass Spectrometry and Double Isotope Labeling Technique.SPECTROSCOPY AND SPECTRAL ANALYSIS,36(5),1478-1482. |
| MLA | Wang Ling-li,et al."The Study of Organic Nitrogen Uptake by Soil Microorganisms Using Gas Chromatography-Mass Spectrometry and Double Isotope Labeling Technique".SPECTROSCOPY AND SPECTRAL ANALYSIS 36.5(2016):1478-1482. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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