Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28
文献类型：期刊论文
| 作者 | Ling, Junhong2; Liu, Yi2,3; Chen, Xiaoqi1,3; Tian, Xiaoyue2,3; Meng, Dali2; Cao, Ting1,3; Pan, Huaqi3; Hu, Jiangchun3; Wang, Nan3 |
| 刊名 | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2018 |
| 卷号 | 82期号:10页码:1832-1839 |
| 关键词 | Cyclopiazonic acid Aspergillus oryzae oxidative burst hybrid pks-nrps plant immune |
| ISSN号 | 0916-8451 |
| DOI | 10.1080/09168451.2018.1490170 |
| 英文摘要 | Extracellular alkalinization and H2O2 production are important early events during induced resistance establishment in plants. In a screen for metabolites as plant resistance activators from 98 fungal isolates associated with marine sponge Hymeniacidon perleve, the cyclopiazonic acids (CPAs) produced by Aspergillus oryzae HMP-F28 induced significant extracellular alkalinization coupled with augmented H2O2 production in tobacco cell suspensions. Bioassay-guided fractionation led to the isolation and structural elucidation of a new CPA congener (4, 3-hydroxysperadine A) and three known ones (1-3). To construct a mutasynthetic strain to generate unnatural CPA analogues, a hybrid pks-nrps gene (cpaS) was disrupted to abolish the production of the critical precursor of cyclo-acetoacetyl-L-tryptophan (cAATrp) and all the downstream CPA products. Elimination of cAATrp will allow cAATrp mimics being processed by the CPA biosynthetic machinery to produce CPA derivatives with designed structural features. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000445240200020 |
| 出版者 | TAYLOR & FRANCIS LTD |
| 源URL | [http://210.72.129.5/handle/321005/123055]  |
| 专题 | 中国科学院沈阳应用生态研究所 |
| 通讯作者 | Wang, Nan |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 2.Shenyang Pharmaceut Univ, Sch Tradit Chinese Mat Med, Shenyang, Liaoning, Peoples R China 3.Chinese Acad Sci, Inst Appl Ecol, Shenyang, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ling, Junhong,Liu, Yi,Chen, Xiaoqi,et al. Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2018,82(10):1832-1839. |
| APA | Ling, Junhong.,Liu, Yi.,Chen, Xiaoqi.,Tian, Xiaoyue.,Meng, Dali.,...&Wang, Nan.(2018).Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,82(10),1832-1839. |
| MLA | Ling, Junhong,et al."Characterization and abolishment of the cyclopiazonic acids produced by Aspergillus oryzae HMP-F28".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 82.10(2018):1832-1839. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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